Mapping with a Few Plants: Using Selective Mapping for Microsatellite Saturation of the Prunus Reference Map

¹ Laboratori de GenLaboratori de Genètica Molecular Vegetaltica Molecular Vegetal. IRTA-CSIC.
² National Institute of Fruit Tree Science,
³ Institut National de la Recherche Agronomique
⁴ University of Udine
⁵ Institut National de la recherche Agronomique
⁶ Clemson University

^* To whom correspondence should be addressed. E-mail: pere.arus{at}irta.es.

Submitted on March 23, 2005
Revised on June 21, 2005
Accepted on 29 July 2005

	Abstract

The concept of selective (or bin) mapping is used here for the first time taking as an example the Prunus reference map constructed with an almond x peach F2 population. Based on this map, a set of six plants was selected that jointly defined 65 possible different genotypes for the codominant markers mapped on it. Sixty-three of these joint genotypes corresponded to a single chromosomal region (a bin) of the Prunus genome, and the two remaining to two bins each. The 67 bins defined by these six plants had 7.8 cM average length and maximum individual length of 24.7 cM. Using a unit of analysis composed of these six plants, their F1 hybrid parent and one of the parents of the hybrid, we mapped 264 microsatellite (or simple-sequence repeat: SSR) markers from 401 different microsatellite primer pairs. Bin mapping proved to be a fast and economic strategy that could be used for further map saturation, the addition of valuable markers, such as those based on microsatellites or ESTs, and to give a wider scope to, and more efficient use of, reference mapping populations.

Key Words: Prunus, SSRs, Selective mapping

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