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doi:10.1534/genetics.105.043125
A more recent version of this article appeared on August 1, 2005.
REGULAR RESEARCH PAPERS |
Identification of Genetic Loci that Interact with cut During Drosophila Wing Margin Development
Joshua J Krupp 1, Lauren E Yaich 2, Robert J Wessells 1 and Rolf Bodmer 1*
1 The Burnham Institute
2 University of Pittsburgh
* To whom correspondence should be addressed. E-mail: rolf{at}burnham.org.
Submitted on March 10, 2005
Revised on April 15, 2005
Accepted on 5 May 2005
The Drosophila selector gene cut is a hierarchal regulator of external sensory organ identity, and is required to pattern the sensory and non-sensory cells of the wing margin. Cut performs the latter function, in part, by maintaining expression of the secreted morphogen encoded by wingless (wg). We find Cut is required for wing margin sensory organ specification in addition to and independent of Wg maintenance. In addition, we performed a genetic modifier screen to identify other genes that interact with cut in the regulation of wing margin patterning. In total, 45 genetic loci (35 gain-of-function and 10 loss-of-function) were identified by virtue of their ability to suppress the wing margin defects resulting from gypsy retrotransposon-mediated insulation of the cut wing margin enhancer. Further genetic characterization identified several subgroups of candidate cut interacting loci. One group consists of putative regulators of gypsy insulator activity. A second group is potentially required for the regulation of Cut expression and/or activity, which includes longitudinals lacking, a gene that encodes a BTB-domain zinc-finger transcription factor. A third group, which includes a component of the Brahma chromatin remodeling complex encoded by moira, affects the level of Cut expression/activity in two opposing ways, by suppressing gypsy-mediated ctK phenotype and enhancing the non-gypsy ct53d phenotype. This suggests that the Brahma complex modulates both enhancer controlled transcription and gypsy-mediated insulation of the cut locus.
Key Words: Drosophila, cut, external sensory organ, selector gene, wing margin
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