- THIS ARTICLE
- Full Text (Rapid PDF)
-
All Versions of this Article:
genetics.105.042598v1
171/1/7 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Umeda, M.
- Articles by Sazer, S.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Umeda, M.
- Articles by Sazer, S.
doi:10.1534/genetics.105.042598
A more recent version of this article appeared on September 1, 2005.
REGULAR RESEARCH PAPERS |
The fission yeast Schizosaccharomyces pombe has two importin-
,
Imp1p and Cut15p, which have common and unique functions in nucleocytoplasmic transport and cell cycle progression
Makoto Umeda 1, Shaded Izaddoost 2, Ian Cushman 3, Mary Shannon Moore 4 and Shelley Sazer 1*
1 Baylor College of Medicine
2 UTMB
3 Duke University
4 Ross University
* To whom correspondence should be addressed. E-mail: ssazer{at}bcm.tmc.edu.
Submitted on February 25, 2005
Revised on March 24, 2005
Accepted on 8 April 2005
The nuclear import of classical Nuclear Localization signal-containing proteins depends on importin-
transport receptors. In budding yeast there is a single importin- gene, in higher eukaryotes there are multiple importin--like genes, but in fission yeast there are two: the previously characterized cut15 and the more recently identified imp1. Like other importin- family members, Imp1p supports nuclear protein import in vitro. In contrast to cut15, imp1 is not essential for viability, but imp1
mutant cells exhibit a telophase delay and mild temperature sensitive lethality. Differences in the cellular functions that depend on Imp1p and Cut15p indicate that they each have unique physiological roles. They also have common roles because: the imp1 and the cut15-85 temperature sensitive mutations are synthetically lethal; overexpression of cut15 partially suppresses the temperature sensitivity, but not the mitotic delay in imp1 cells; and overexpression of imp1 partially suppresses the mitotic defect in cut15-85 cells but not the loss of viability. Imp1p and Cut15p are both required for the efficient nuclear import of both an SV40 nuclear localization signal containing reporter protein and the Pap1p component of the stress response MAP kinase pathway. Imp1p and Cut15p are essential for efficient nuclear protein import in S. pombe.
Key Words: S. pombe, cell cycle, fission yeast, importin-alpha, nucleocytoplasmic transport
This article has been cited by other articles:
 |
|
 |
|
  N. Nakazawa, T. Nakamura, A. Kokubu, M. Ebe, K. Nagao, and M. Yanagida Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis J. Cell Biol., March 24, 2008; 180(6): 1115 - 1131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sistla, J. V. Pang, C. X. Wang, and D. Balasundaram Multiple Conserved Domains of the Nucleoporin Nup124p and Its Orthologs Nup1p and Nup153 Are Critical for Nuclear Import and Activity of the Fission Yeast Tf1 Retrotransposon Mol. Biol. Cell, September 1, 2007; 18(9): 3692 - 3708. [Abstract] [Full Text] [PDF]
|
|