Genetics. Published Articles Ahead of Print: June 18, 2005, Copyright © 2005
doi:10.1534/genetics.105.042218


A more recent version of this article appeared on September 1, 2005.

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RESISTANCE TO FUSARIUM OXYSPORUM 1, a Dominant Arabidopsis Resistance Gene, is not Race Specific

andrew C Diener 1 and Frederick M. Ausubel 1*

1 Massachusetts General Hospital

* To whom correspondence should be addressed. E-mail: ausubel{at}molbio.mgh.harvard.edu.

Submitted on February 22, 2005
Revised on April 23, 2005
Accepted on 13 May 2005


Abstract

Arabidopsis thaliana ecotypes differ in their susceptibility to Fusarium wilt diseases. Ecotype Taynuilt-0 (Ty-0) is susceptible to Fusarium oxysporum forma specialis (f.) matthioli whereas Columbia-0 (Col-0) is resistant. Segregation analysis of a cross between Ty-0 and Col-0 revealed six dominant RESISTANCE TO FUSARIUM OXYSPORUM (RFO) loci that significantly contribute to f. matthioli resistance in Col-0 relative to Ty-0. We refer to the locus with the strongest effect as RFO1. Ty-0 plants in which only the Col-0 allele of RFO1 (RFO1Col-0) was introduced were resistant to f. matthioli. Surprisingly, RFO1Col-0 also conferred resistance to f. raphani, demonstrating that RFO1-mediated resistance is not race specific. Expression of resistance by RFO2, RFO4 or RFO6 was dependent on RFO1Col-0. Map based cloning of RFO1Col-0 showed that RFO1 is identical to the previously named Arabidopsis gene WAKL22 (WALL-ASSOCIATED KINASE-LIKE KINASE 22), which encodes a receptor-like kinase that does not contain an extracellular leucine-rich repeat domain. Consistent with these results, a Col-0 rfo1 loss-of-function mutant was more susceptible to f. matthioli, f. conglutinans and f. raphani. Thus, RFO1 encodes a novel type of dominant disease resistance protein that confers resistance to a broad spectrum of Fusarium races.

Key Words: Arabidopsis thaliana, Fusarium oxysporum, RFO1, WAKL22, disease resistance




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