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doi:10.1534/genetics.105.041749
A more recent version of this article appeared on August 1, 2005.
Originally published as Genetics Published Articles Ahead of Print on June 14, 2005.
REGULAR RESEARCH PAPERS |
Adult myogenesis in Drosophila melanogaster can proceed independently of myocyte enhancer factor-2
Phill W Baker 1, Kathleen Kelly Tanaka 1, Niels Klitgord 1 and Richard M Cripps 1*
1 University of New Mexico
* To whom correspondence should be addressed. E-mail: rcripps{at}unm.edu.
Submitted on February 7, 2005
Revised on March 11, 2005
Accepted on 6 May 2005
Myocyte Enhancer Factor-2 (MEF2) is a transcription factor that is necessary for embryonic muscle development in Drosophila and vertebrates, however the requirement for this factor during later muscle development remains largely unknown. Using heteroallelic combinations of different Mef2 mutant alleles, we isolated and characterized a temperature sensitive combination. Through temperature shift experiments, we obtained adult animals that were lacking proper MEF2 function. Many of these individuals died as mature pupae, and those that eclosed showed poor locomotion and an inability to fly. Histological analysis of these animals revealed a requirement for MEF2 in skeletal muscle patterning, although these animals had strikingly normal amounts of muscle tissue. Using quantitative polymerase chain reaction, we determined that expression of the MEF2-regulated actin gene Act57B was severely reduced in these animals. By contrast myofibrillar actin genes unique to the adult stage were only mildly affected. Since MEF2 mutant adults were still capable of forming muscle tissue, we conclude that MEF2 is required for the expression of only a subset of adult muscle structural genes in the adult. These results indicate that additional muscle-specific factors function to control the myogenesis of complex and diverse muscle in the adult.
Key Words: Drosophila, actin, muscle, myocyte enhancer factor 2, transcription
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