- THIS ARTICLE
- Full Text (Rapid PDF)
- Supplemental Data
-
All Versions of this Article:
genetics.105.041574v1
170/2/631 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Kim, K.-S.
- Articles by Inwood, W.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Kim, K.-S.
- Articles by Inwood, W.
doi:10.1534/genetics.105.041574
A more recent version of this article appeared on June 1, 2005.
REGULAR RESEARCH PAPERS |
Spontaneous Mutations in the Ammonium Transport Gene AMT4 of Chlamydomonas reinhardtii
Kwang-Seo Kim 1, Eithne Feild 1, Natalie King 1, Takuro Yaoi 1, Sydney Kustu 1* and William Inwood 1
1 University of California, Berkeley
* To whom correspondence should be addressed. E-mail: kustu{at}nature.berkeley.edu.
Submitted on February 7, 2005
Revised on February 23, 2005
Accepted on 23 February 2005
Evidence in several microorganisms indicates that Amt proteins are gas channels for NH3 and CH3NH2, and this has been confirmed structurally. Chlamydomonas reinhardtii has at least four AMT genes, the most reported for a microorganism. Under nitrogen-limiting conditions all AMT genes are transcribed and Chlamydomonas is sensitive to methylammonium toxicity. All 16 spontaneous methylammonium-resistant mutants we analyzed had defects in accumulation of [14C]methylammonium. Genetic crosses indicated that 12 had lesions in a single locus, whereas two each had lesions in other loci. Lesions in different loci were correlated with different degrees of defect in [14C]methylammonium uptake. One mutant in the largest class had an insert in the AMT4 gene, and the insert co-segregated with methylammonium resistance in genetic crosses. The other 11 strains in this class also had amt4 lesions, which we characterized at the molecular level. Properties of the amt4 mutants were clearly different from those of rh1 RNAi lines. They indicated that the physiological substrates for Amt and Rh proteins, the only two members of their protein superfamily, are NH3 and CO2, respectively.
Key Words: AMT genes, Chlamydomonas reinhardtii, RH genes, gas channels, transposition
This article has been cited by other articles:
 |
|
 |
|
  B. Cherif-Zahar, A. Durand, I. Schmidt, N. Hamdaoui, I. Matic, M. Merrick, and G. Matassi Evolution and Functional Characterization of the RH50 Gene from the Ammonia-Oxidizing Bacterium Nitrosomonas europaea J. Bacteriol., December 15, 2007; 189(24): 9090 - 9100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Fernandez and A. Galvan Inorganic nitrogen assimilation in Chlamydomonas J. Exp. Bot., July 1, 2007; 58(9): 2279 - 2287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-S. Kim, S. Kustu, and W. Inwood Natural History of Transposition in the Green Alga Chlamydomonas reinhardtii: Use of the AMT4 Locus as an Experimental System Genetics, August 1, 2006; 173(4): 2005 - 2019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Ji, S. Hashmi, Z. Liu, J. Zhang, Y. Chen, and C.-H. Huang CeRh1 (rhr-1) is a dominant Rhesus gene essential for embryonic development and hypodermal function in Caenorhabditis elegans PNAS, April 11, 2006; 103(15): 5881 - 5886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Huang and J. Peng Evolutionary conservation and diversification of Rh family genes and proteins PNAS, October 25, 2005; 102(43): 15512 - 15517. [Abstract] [Full Text] [PDF]
|
|