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doi:10.1534/genetics.105.040584
A more recent version of this article appeared on September 1, 2005.
REGULAR RESEARCH PAPERS |
The heterotrimeric G-protein GanB(
)-SfaD(
)-GpgA(
) is a carbon source sensor involved in early cAMP-dependent germination in Aspergillus nidulans
Anne Lafon 1, Jeong-Ah Seo 2, Kap-Hoon Han 2, Jae-Hyuk Yu 2 and Christophe d'Enfert 1*
1 Institut Pasteur
2 University of Wisconsin-Madison
* To whom correspondence should be addressed. E-mail: denfert{at}pasteur.fr.
Submitted on January 6, 2005
Revised on February 25, 2005
Accepted on 11 April 2005
The role of heterotrimeric G-proteins in cAMP-dependent germination of conidia was investigated in the filamentous ascomycete Aspergillus nidulans. We demonstrate that the G
subunit GanB mediates a rapid and transient activation of cAMP synthesis in response to glucose during the early period of germination. Moreover, deletion of individual G protein subunits resulted in defective trehalose mobilization and altered germination kinetics, indicating that GanB()-SfaD(
)-GpgA(
constitute a functional heterotrimer and control cAMP/PKA signaling in response to glucose as well as conidial germination. Further genetic analyses suggest that GanB plays a primary role in cAMP/PKA signaling, whereas the SfaD-GpgA (G) hetero-dimer is crucial for proper activation of GanB signaling sensitized by glucose. In addition, the RGS protein RgsA is also involved in regulation of the cAMP/PKA pathway and germination via attenuation of GanB signaling. Genetic epistatic analyses led us to conclude that all controls exerted by GanB()-SfaD()-GpgA( on conidial germination are mediated through the cAMP/PKA pathway. Furthermore, GanB may function in sensing various carbon sources and subsequent activation of downstream signaling for germination.
Key Words: RGS protein, cAMP/PKA signaling, conidial germination, heterotrimeric G-proteins
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