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doi:10.1534/genetics.104.039750
A more recent version of this article appeared on June 1, 2005.
REGULAR RESEARCH PAPERS |
A screen for genes that influence FGF signal transduction in Drosophila
Min Yan Zhu 1, Robert Wilson 2 and Maria Leptin 2*
1 Institut für Neurobiologie Universität zu Münster
2 Institut fuer Genetik der Universitaet zu Koeln
* To whom correspondence should be addressed. E-mail: mleptin{at}uni-koeln.de.
Submitted on December 13, 2004
Revised on February 25, 2005
Accepted on 25 February 2005
The mis-expression of an activated form of the FGF receptor (FGFR) Breathless in conjunction with the signaling molecule Downstream-of-FGF-receptor (Dof), which is a cytoplasmic protein containing a DBB domain, two ankyrin repeats and a coiled coil, in the Drosophila eye imaginal discs impairs eye development and results in a rough eye phenotype. We have used this phenotype in a gain-of-function screen to search for modifiers of FGF signaling. We identified 50 EP stocks with insertions defining at least 35 genes that affect the rough eye phenotype. Amongst these genes, 4 appeared to be specific for FGFR signaling, but most of the genes also influenced other signaling pathways, as assessed by their effects on rough eyes induced by other activated receptor tyrosine kinases (RTKs). Analysis of loss of function alleles of a number of these genes in embryos indicates that in many cases the products are provided maternally and are involved in germ cell development. At least two of the genes, sar1 and robo2, show a genetic interaction with a hypomorphic dof allele suggesting that they participate in FGF-mediated morphogenetic events during embryogenesis.
Key Words: Formin binding protein 11, Robo 2, Sar1, VRK1, tracheal development
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