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doi:10.1534/genetics.104.039479
A more recent version of this article appeared on May 1, 2005.
REGULAR RESEARCH PAPERS |
Maternal Transmission Ratio Distortion at the Mouse Om Locus Results from Meiotic Drive at the Second Meiotic Division
Guangming Wu 1, Lanping Hao 1, Zhiming Han 1, Shaorong Gao 1, Keith E. Latham 1, Fernando Pardo-Manuel de Villena 2 and Carmen Sapienza 1*
1 Fels Institute for Cancer Research
2 University of North Carolina, Chapel Hill
* To whom correspondence should be addressed. E-mail: sapienza{at}temple.edu.
Submitted on December 9, 2004
Revised on January 6, 2005
Accepted on 26 January 2005
We have observed maternal transmission ratio distortion (TRD) in favor of DDK alleles at the Ovum mutant (Om) locus on mouse chromosome 11 among the offspring of (C57BL/6 X DDK)F1 females and C57BL/6 males. Although significant lethality occurs in this backcross (approximately 50%), differences in the level of TRD found in recombinant versus non-recombinant chromosomes among offspring argues that TRD is due to non-random segregation of chromatids at the second meiotic division; i.e., true meiotic drive. We tested this hypothesis directly, by determining the centromere and Om genotypes of individual chromatids in zygote stage embryos. We found similar levels of TRD in favor of DDK alleles at Om in the female pronucleus and TRD in favor of C57BL/6 alleles at Om in the second polar body. In those embryos for which complete dyads have been reconstructed, TRD was present only in those inheriting heteromorphic dyads. These results demonstrate that meiotic drive occurs at MII and that preferential death of one genotypic class of embryo does not play a large role in the TRD.
Key Words: meiotic drive, nested PCR, polar body, pronucleus, transmission ratio distortion
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