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doi:10.1534/genetics.104.037085
A more recent version of this article appeared on February 1, 2005.
REGULAR RESEARCH PAPERS |
Bonus, a Drosophila TIF1 homologue, is a chromatin associated protein that acts as a modifier of position effect variegation
Robert B. Beckstead 1, Sarbjit S. Ner 2, Karen G. Hales 3, Thomas A. Grigliatti 2, Bruce S. Baker 4 and Hugo J. Bellen 5*
1 University of Utah
2 University of British Columbia
3 Davidson College
4 Stanford University
5 Baylor College of Medicine
* To whom correspondence should be addressed. E-mail: hbellen{at}bcm.tmc.edu.
Submitted on October 1, 2004
Revised on November 8, 2004
Accepted on 8 November 2004
Bonus, a Drosophila TIF1 homolog, is a nuclear receptor cofactor that is required for viability, molting, and numerous morphological events. Here we establish a role for Bonus in the modulation of chromatin structure. We show that weak loss-of-function alleles of bonus have a more deleterious effect on males than females. This male-enhanced lethality is not due to a defect in dosage compensation or somatic sex differentiation, but the presence of the Y chromosome. Additionally, we show that bonus acts as both an enhancer and suppressor of position effect variegation. By immunostaining, we demonstrate that Bonus is associated with both interphase and prophase chromosomes and through chromatin immunoprecipitation show that two of these sites correspond to the Histone gene cluster and the Stellate locus.
Key Words: enhancer of variegation, heterochromatin, position-effect variegation, suppressor of variegation, transcription
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