- THIS ARTICLE
- Full Text (Rapid PDF)
-
All Versions of this Article:
genetics.104.036772v1
170/3/1091 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
-
Author home page(s):
Heather H. Wilkinson
Daniel J. Ebbole
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Li, D.
- Articles by Ebbole, D. J.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Li, D.
- Articles by Ebbole, D. J.
doi:10.1534/genetics.104.036772
A more recent version of this article appeared on July 1, 2005.
REGULAR RESEARCH PAPERS |
A MAP Kinase Pathway Essential for Mating and Contributing to Vegetative Growth in Neurospora crassa
Dan Li 1, Piotr Bobrowicz 1, Heather H. Wilkinson 1 and Daniel J. Ebbole 1*
1 Texas A&M University
* To whom correspondence should be addressed. E-mail: d-ebbole{at}neo.tamu.edu.
Submitted on September 25, 2004
Revised on October 28, 2004
Accepted on 15 February 2005
MAP kinases homologous to Saccharomyces cerevisiae Fus3p/Kss1p have been identified in plant pathogenic fungi and are required for pathogenicity and sexual reproduction. To better understand the role of MAP kinase signaling in Neurospora crassa, and to identify downstream target genes of the pathway, we isolated, cloned, and disrupted the FUS3 homolog mak-2. Ste12p is a transcription factor target of Fus3p that activates genes of the mating pathway in yeast, and we also characterized the N. crassa STE12 homolog, pp-1. The mak-2 and pp-1 mutants have reduced growth rate, produce short aerial hyphae, and fail to develop protoperithecia. In addition, ascospores carrying null mutations of either gene are inviable. Subtractive cloning was used to isolate genes having reduced expression in the mak-2 mutant. Expression of some of these genes is protoperithecia-specific and three of them are part of a gene cluster potentially involved in the production of a polyketide secondary metabolite. Microarray analysis was used to extend the analysis of gene expression in mak-2 and pp-1 mutants. The role of the MAP kinase pathway in both sexual and asexual development as well as secondary metabolism is consistent with the dual regulation of the mating process and pathogencity observed in fungal pathogens.
Key Words: MAP kinase, microarray, polyketide synthase, protoperithecia, subtractive cloning
This article has been cited by other articles:
 |
|
 |
|
  S. Maerz, C. Ziv, N. Vogt, K. Helmstaedt, N. Cohen, R. Gorovits, O. Yarden, and S. Seiler The Nuclear Dbf2-Related Kinase COT1 and the Mitogen-Activated Protein Kinases MAK1 and MAK2 Genetically Interact to Regulate Filamentous Growth, Hyphal Fusion and Sexual Development in Neurospora crassa Genetics, July 1, 2008; 179(3): 1313 - 1325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Atoui, D. Bao, N. Kaur, W. S. Grayburn, and A. M. Calvo Aspergillus nidulans Natural Product Biosynthesis Is Regulated by MpkB, a Putative Pheromone Response Mitogen-Activated Protein Kinase Appl. Envir. Microbiol., June 1, 2008; 74(11): 3596 - 3600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yi, N. Sahni, K. J. Daniels, C. Pujol, T. Srikantha, and D. R. Soll The Same Receptor, G Protein, and Mitogen-activated Protein Kinase Pathway Activate Different Downstream Regulators in the Alternative White and Opaque Pheromone Responses of Candida albicans Mol. Biol. Cell, March 1, 2008; 19(3): 957 - 970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhao, R. Mehrabi, and J.-R. Xu Mitogen-Activated Protein Kinase Pathways and Fungal Pathogenesis Eukaryot. Cell, October 1, 2007; 6(10): 1701 - 1714. [Full Text] [PDF]
|
|
|
|
|
|
C. A. Jones, S. E. Greer-Phillips, and K. A. Borkovich The Response Regulator RRG-1 Functions Upstream of a Mitogen-activated Protein Kinase Pathway Impacting Asexual Development, Female Fertility, Osmotic Stress, and Fungicide Resistance in Neurospora crassa Mol. Biol. Cell, June 1, 2007; 18(6): 2123 - 2136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Deng, T. D. Allen, and D. L. Nuss Ste12 Transcription Factor Homologue CpST12 Is Down-Regulated by Hypovirus Infection and Required for Virulence and Female Fertility of the Chestnut Blight Fungus Cryphonectria parasitica Eukaryot. Cell, February 1, 2007; 6(2): 235 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Breakspear and M. Momany The first fifty microarray studies in filamentous fungi Microbiology, January 1, 2007; 153(1): 7 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. V. Colot, G. Park, G. E. Turner, C. Ringelberg, C. M. Crew, L. Litvinkova, R. L. Weiss, K. A. Borkovich, and J. C. Dunlap A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors PNAS, July 5, 2006; 103(27): 10352 - 10357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Ren, D. J. Springer, M. J. Behr, W. A. Samsonoff, S. Chaturvedi, and V. Chaturvedi Transcription Factor STE12{alpha} Has Distinct Roles in Morphogenesis, Virulence, and Ecological Fitness of the Primary Pathogenic Yeast Cryptococcus gattii. Eukaryot. Cell, July 1, 2006; 5(7): 1065 - 1080. [Abstract] [Full Text] [PDF]
|
|