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doi:10.1534/genetics.104.035980
A more recent version of this article appeared on February 1, 2005.
NOTE |
Chromosome identification and nomenclature of Sorghum bicolor
Jeong-Soon Kim 1, Patricia E. Klein 1, Robert R. Klein 2, H. James Price 1, John E. Mullet 1 and David M. Stelly 1*
1 Texas A&M University
2 USDA-ARS
* To whom correspondence should be addressed. E-mail: stelly{at}tamu.edu.
Submitted on September 6, 2004
Revised on September 21, 2004
Accepted on 1 October 2004
Linkage group identities and homologies were determined for metaphase chromosomes of Sorghum bicolor (2n=20) by FISH of landed BACs. Relative lengths of chromosomes in FISH-karyotyped metaphase spreads of the elite inbred BTx623 were used to estimate the molecular size of each chromosome, and to establish a size-based nomenclature for sorghum chromosomes (SBI-01 to SBI-10) and linkage groups (LG-01 to LG-10). Lengths of arms were determined to orient linkage groups relative to a standard karyotypic layout (short arms at top). The size-based nomenclature for BTx623 represents a reasonable choice as the standard for a unified chromosome nomenclature for use by the sorghum research community.
Key Words: FISH, genome, karyotype, map, sorghum
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