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doi:10.1534/genetics.104.035873
A more recent version of this article appeared on January 1, 2005.
REGULAR RESEARCH PAPERS |
Genome-wide screen for genes with effects on distinct iron uptake activities in Saccharomyces cerevisiae
Emmanuel Lesuisse 1*, Simon A.B. Knight 2, Maite Courel 3, Renata Santos 3, Jean-Michel Camadro 3 and Andrew Dancis 2
1 Institut Jacques Monod, CNRS
2 University of Pennsylvania
3 Institut Jacques Monod
* To whom correspondence should be addressed. E-mail: lesuisse{at}ijm.jussieu.fr.
Submitted on September 3, 2004
Revised on October 6, 2004
Accepted on 6 October 2004
We screened a collection of 4847 haploid knockout strains (EUROSCARF collection) of Saccharomyces cerevisiae for iron uptake from the siderophore ferrioxamine B (FOB). A large number of mutants showed altered uptake activities, and a few turned yellow when grown on agar plates with added FOB, indicating increased intracellular accumulation of undissociated siderophore. A subset consisting of 197 knockouts with altered uptake were examined further for regulated activities that mediate cellular uptake of iron from other siderophores or from iron salts. Hierarchical clustering analysis grouped the data according to iron sources and according to mutant categories. In the first analysis, siderophores grouped together with the exception of enterobactin, which grouped with iron salts, suggesting a reductive pathway of iron uptake for this siderophore. Mutant groupings included three categories: i) high FOB uptake, high reductase, low ferrous transport; ii) isolated high or low FOB transport; and iii) induction of all activites. Mutants with statistically altered uptake activities included genes encoding proteins with predominant localization in the secretory pathway, nucleus and mitochondria. Measurements of different iron uptake activites in the yeast knockout collection makes possible distinctions between genes with general effects on iron metabolism and those with pathway specific effects.
Key Words: genome, iron, siderophore, yeast
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