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doi:10.1534/genetics.104.034322
A more recent version of this article appeared on May 1, 2005.
REGULAR RESEARCH PAPERS |
Mutations in the Saccharomyces cerevisae LSM1 gene that affect mRNA decapping and 3' end protection
Tharun Sundaresan 1*, Ashis Chowdhury 1, Denise Muhlrad 2 and Roy Parker 2
1 Uniformed Services University of the Health Sciences (USUHS)
2 The University of Arizona
* To whom correspondence should be addressed. E-mail: tsundaresan{at}usuhs.mil.
Submitted on August 6, 2004
Revised on October 17, 2004
Accepted on 18 January 2005
The decapping of eukaryotic mRNAs is a key step in their degradation. The heteroheptameric Lsm1p-7p complex is a general activator of decapping and also functions in protecting the 3' ends of deadenylated mRNAs from a 3' trimming reaction. Lsm1p is the unique member of the Lsm1p-7p complex distinguishing that complex from the functionally different Lsm2p-8p complex. To understand the function of Lsm1p, we constructed a series of deletion and point mutants of the LSM1 gene and examined their phenotypes. These studies revealed the following. (i) Integrity of the Lsm1p-7p complex is important for its mRNA decay function since mutations affecting the predicted inter-subunit interaction surfaces of Lsm1p lead to impairment of mRNA decay. (ii) Interactions between Lsm1p and mRNA are necessary for the activation of decapping by the Lsm1p-7p complex. However, even when these interactions are disrupted, the Lsm1p-7p complex could localize to the P-bodies. (iii) 3' end protection could be indicative of the binding of the Lsm1p-7p complex to the mRNA prior to activation of decapping since all the mutants defective in mRNA 3' end protection were also blocked in mRNA decay. (iv) In addition to the Sm domain the C-terminal domain of Lsm1p is also important for mRNA decay function.
Key Words: Lsm1, Sm-like, decapping, decay, mRNA
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