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doi:10.1534/genetics.104.031146
A more recent version of this article appeared on January 1, 2005.
Regular Research Papers |
Molecular cytogenetic characterization of the Antirrhinum majus genome
Dongfen Zhang 1, Qiuying Yang 1, Weidong Bao 1, Yu Zhang 2, Bin Han 2, Yongbiao Xue 1 and Zhukuan Cheng 1*
1 Institute of Genetics and Developmental Biology,CAS
2 National Center for Gene Research, Shanghai Institutes for Biological Sciences,CAS
* To whom correspondence should be addressed. E-mail: zkcheng{at}genetics.ac.cn.
Submitted on May 11, 2004
Revised on July 7, 2004
Accepted on 1 September 2004
As a model system in classical plant genetics, the genus Antirrhinum has been well studied, especially in gametophytic self-incompacibility, flower development biology and transposon induced mutation. In contrast to the advances in genetic and molecular studies, little is known about Antirrhinum cytogenetics. In the present study, we isolated two tandem repetitive sequences, CentA1 and CentA2, from the centromeric regions of Antirrhinum chromosomes. A standard karyotype has been established by anchoring these centromeric repeats on meiotic pachytene chromosome using FISH. An ideogram based on the DAPI staining pattern of pachytene chromosomes was developed to depict the distribution of heterochromatin in the Antirrhinum majus genome. To integrate the genetic and chromosomal maps, we selected 1-2 molecular markers from each linkage group to screen an Antirrhinum TAC library. These genetically anchored TAC clones were labeled as FISH probes to hybridize to pachytene chromosomes of A. majus. As a result, the relationship between chromosomes and the linkage groups (LGs) in Antirrhinum has been established.
Key Words: Antirrhinum, FISH, chromosome
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