- THIS ARTICLE
- Full Text
- Full Text (PDF)
-
All Versions of this Article:
genetics.105.054429v1
173/1/389 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Goel, S.
- Articles by Ozias-Akins, P.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Goel, S.
- Articles by Ozias-Akins, P.
Originally published as Genetics Published Articles Ahead of Print on March 17, 2006.
Genetics, Vol. 173, 389-400, May 2006, Copyright © 2006
doi:10.1534/genetics.105.054429
Comparative Physical Mapping of the Apospory-Specific Genomic Region in Two Apomictic Grasses: Pennisetum squamulatum and Cenchrus ciliaris
Shailendra Goel*,
Zhenbang Chen*,1,2,
Yukio Akiyama*,1,3,
Joann A. Conner*,
Manojit Basu*,4,
Gustavo Gualtieri*,5,
Wayne W. Hanna
and
Peggy Ozias-Akins*,6
* Department of Horticulture, University of Georgia Tifton Campus, Tifton, Georgia 31793-0748 and Department of Crop and Soil Sciences, University of Georgia Tifton Campus, Tifton, Georgia 31793-0748
6 Corresponding author: Department of Horticulture, University of Georgia Tifton Campus, 2356 Rainwater Rd., Tifton, GA 31794.
E-mail: pozias{at}uga.edu
In gametophytic apomicts of the aposporous type, each cell of the embryo sac is genetically identical to somatic cells of the ovule because they are products of mitosis, not of meiosis. The egg of the aposporous embryo sac follows parthenogenetic development into an embryo; therefore, uniform progeny result even from heterozygous plants, a trait that would be valuable for many crop species. Attempts to introgress apomixis from wild relatives into major crops through traditional breeding have been hindered by low or no recombination within the chromosomal region governing this trait (the apospory-specific genomic region or ASGR). The lack of recombination also has been a major obstacle to positional cloning of key genes. To further delineate and characterize the nonrecombinant ASGR, we have identified eight new ASGR-linked, AFLP-based molecular markers, only one of which showed recombination with the trait for aposporous embryo sac development. Bacterial artificial chromosome (BAC) clones identified with the ASGR-linked AFLPs or previously mapped markers, when mapped by fluorescence in situ hybridization in Pennisetum squamulatum and Cenchrus ciliaris, showed almost complete macrosynteny between the two apomictic grasses throughout the ASGR, although with an inverted order. A BAC identified with the recombinant AFLP marker mapped most proximal to the centromere of the ASGR-carrier chromosome in P. squamulatum but was not located on the ASGR-carrier chromosome in C. ciliaris. Exceptional regions where synteny was disrupted probably are nonessential for expression of the aposporous trait. The ASGR appears to be maintained as a haplotype even though its position in the genome can be variable.
This article has been cited by other articles:
 |
|
 |
|
  J. A. Conner, S. Goel, G. Gunawan, M.-M. Cordonnier-Pratt, V. E. Johnson, C. Liang, H. Wang, L. H. Pratt, J. E. Mullet, J. DeBarry, et al. Sequence Analysis of Bacterial Artificial Chromosome Clones from the Apospory-Specific Genomic Region of Pennisetum and Cenchrus Plant Physiology, July 1, 2008; 147(3): 1396 - 1411. [Abstract] [Full Text] [PDF]
|
|