Collaboration Between the Essential Esa1 Acetyltransferase and the Rpd3 Deacetylase Is Mediated by H4K12 Histone Acetylation in Saccharomyces cerevisiae
Genetics Chang and Pillus 183: 149

Supporting Information for Chang and Pillus, 2009

Supporting Information

Supporting Information - File S1, Figures S1-S6, and Tables S1-S4 (PDF, 620 KB)
Figure S1 - Suppression of esa1’s growth defect by rpd3 was not allele-specific (PDF, 104 KB)
Figure S2 - RPD3 mutant lacking catalytic activity suppresses silencing defects of esa1 (PDF, 112 KB)
Figure S3 - Mutant phenotypes of rpd3 are not suppressed by esa1 mutation (PDF, 168 KB)
Figure S4 - Deletion of non-catalytic subunits of Rpd3 complexes does not change global acetylation of H4K5 or H4K12 (PDF, 52 KB)
Figure S5 - Growth rescue of esa1 mutant by catalytcally inactive rpd3-H150A-H151A is dependent on H4K12 (PDF, 96 KB)
Figure S6 - rDNA and telomeric silencing suppression is not dependent on a single H4 lysine (PDF, 256 KB)
Table S1 - Yeast strains used in this study (PDF, 96 KB)
Table S2 - Plasmids used in this study (PDF, 60 KB)
Table S3 - Oligonucleotide sequences used in this study (PDF, 60 KB)
Table S4 - Summary of Results: effects of second mutation on esa1 mutant (PDF, 68 KB)