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Department of Biology, McGill University, Montreal, Quebec H3A 1B1, Canada
2 Corresponding author: Department of Biology, 1205 Ave. Docteur Penfield, Montreal, Quebec H3A 1B1, Canada.
E-mail: siegfried.hekimi{at}mcgill.ca
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ABSTRACT |
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 TOP ABSTRACT MATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSLITERATURE CITED |
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One way to manipulate ROS levels in vivo, and to test their effects on the life span of intact organisms, is by altering the expression of genes that code for proteins involved in ROS production or detoxification. This strategy has been followed in a variety of organisms, in particular by knocking out, or overexpressing, detoxifying enzymes such as superoxide dismutases (SOD) (PHILLIPS et al. 1989; PARKES et al. 1999; MELOV et al. 2001; SUN et al. 2002; DUTTAROY et al. 2003; FABRIZIO et al. 2003; HARRIS et al. 2003). In C. elegans, there are five distinct genes that code for SODs: sod-2 and sod-3 encode MnSODs that are localized in the mitochondrial matrix (HUNTER et al. 1997), sod-1 encodes the classical cytoplasm CuZnSOD (GIGLIO et al. 1994), sod-4 encodes a membrane-bound and extracellular CuZn SOD (FUJII et al. 1998), and sod-5 encodes a relatively uncharacterized, alternative cytoplasmic CuZnSOD (JENSEN and CULOTTA 2005). We focus on sod-1 and sod-2 because they encode the primary and the best-characterized superoxide dismutases of the two categories: mitochondrial matrix MnSODs and CuZnSODs.
Most, or all, long-lived C. elegans mutants display other phenotypes in addition to increased life span (HEKIMI et al. 2001a,b; HEKIMI and GUARENTE 2003). To test whether the increased life span of these mutants could be due to low ROS levels, we have investigated whether any of the phenotypes of long-lived mutants, including their life span, were correlated with the expression of SODs or could be suppressed by knocking down the SODs by RNAi. We focused on strains carrying one or several mutations in four well-characterized genes whose alteration can result in increased longevity and whose interactions have been previously studied. daf-2 encodes an insulin-receptor-like tyrosine kinase (KENYON et al. 1993); clk-1 encodes a ubiquinone biosynthetic enzyme (EWBANK et al. 1997; STENMARK et al. 2001) and clk-1 mutants display respiratory defects (FELKAI et al. 1999; MIYADERA et al. 2001; BRAECKMAN et al. 2002a,b; KAYSER et al. 2004); isp-1 encodes the "Rieske" iron–sulfur protein (FENG et al. 2001), a catalytic subunit of mitochondrial complex III; and ctb-1, a gene encoded by the mitochondrial genome, encodes the cytochrome b of complex III (FENG et al. 2001). Mutations in daf-2 and clk-1 act synergistically to induce a very long life span (LAKOWSKI and HEKIMI 1996). In contrast, mutations in isp-1 and daf-2 are nonadditive (FENG et al. 2001), suggesting that mutations in these genes affect life span by a mechanism that is similar, although loss-of-function mutations in daf-16, which abolish the increased life span of daf-2 mutants, do not abolish the increased life span of isp-1 (FENG et al. 2001) or isp-1 daf-2 mutants (J. FENG and S. HEKIMI, unpublished observations). ctb-1(qm189) strongly suppresses many of the isp-1 phenotypes, including slow development and behavior, but does not suppress the increased life span (FENG et al. 2001).
It is often difficult to interpret the effect of life-span-shortening treatments because a deleterious treatment could shorten life span without acting on the actual aging process. However, it is possible to exclude a major role in life-span determination of a particular molecular process (here superoxide detoxification) when a treatment is well defined and its molecular and phenotypic effects can be directly observed, yet the process does not impinge significantly on life span. Here we show that the increased life spans of mutants that have altered mitochondrial function and/or increased resistance to oxidative stress are not affected by reductions in superoxide detoxification and concomitant increases in oxidative damage.
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MATERIALS AND METHODS |
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TOPABSTRACTMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSLITERATURE CITED |
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Generation of sod-1 and sod-2 RNAi clones:
Full-length sod-1 and sod-2 cDNA sequences were obtained by RT–PCR and were cloned into the L4440 feeding vector (pPD129.36). The resulting plasmids were transformed into bacteria DH10b and then retransformed into the RNase III-deficient feeding strain HT115 (DE3) (SHIBATA et al. 2003). Primer sequences can be obtained upon request.
Feeding RNAi:
Single colonies of HT115 bacteria were inoculated in LB broth containing 50 µg/ml ampicillin (Amp) and grown overnight at 37°. Overnight cultures were diluted 1:100 and allowed to grow for 6–8 hr and then seeded directly onto NGM plates with Amp and 1 mM IPTG. Seeded plates were dried and induced overnight at room temperature. Animals that were grown on bacteria transformed with the empty vector were used as control. The technique used is essentially as described (KAMATH et al. 2001).
Post-embryonic development:
L4 stage hermaphrodite worms (P0) were placed onto newly prepared (fresh) RNAi plates and were incubated at 20° for 24 hr. Then young adults were transferred to fresh RNAi plates and were incubated for 24–48 hr to lay eggs. Unstaged eggs were placed onto RNAi plates and left for 3 hr to hatch. Larvae (F1 progeny) that had hatched during that period were placed onto fresh RNAi plates (10/plate for a total of 50) at 20° and were monitored every 1.5 or 3 hr until maturity.
Life span:
The aging experiments were begun as in post-embryonic development assays. F1 worms were cultured on RNAi plates at 20° and examined every day until death. Animals were transferred to fresh RNAi plates twice each week.
Brood size:
F1 worms were placed singly onto fresh RNAi plates at 20° until they had matured and begun to lay eggs. The hermaphrodites then were transferred to RNAi plates daily and the progeny were counted. Worms that did not lay any eggs were defined as sterile.
Embryonic lethality:
Eggs produced by F1 worms during a limited time period (4–6 hr) were placed onto fresh RNAi plates and were monitored every day until hatching. The eggs that did not hatch were scored as dead embryos.
Paraquat resistance:
Groups of L4 larvae were grown on control as well as on sod-1 and sod-2 RNAi plates until their progeny had reached the L4 stage. A total of 50 of those were transferred onto NGM plates with 4 mM paraquat (PQ) and kept for 72 hr at 20°, at which time the number of surviving worms was counted.
Paraquat-induced damage to proteins:
L1 wild-type worms were transferred onto plates with different concentrations of paraquat (0.0, 0.1, 0.2, or 0.5 mM) and left to develop into adults. Their adult progeny was then harvested and used for protein extraction by freeze–thaw and dissolved in NET buffer. Protein concentrations were measured with the Bio-Rad (Hercules, CA) kit and then normalized to 5 µg/µl and used with the Oxyblot (Chemicon) kit and analyzed as described below.
Antibodies:
Whole cDNA sequences of sod-1 and sod-2 were amplified from a C. elegans cDNA library (Invitrogen, San Diego) and cloned into the pGEX-5X-1 vector (Invitrogen). Bacterially expressed GST-fusion proteins were extracted and injected into two rabbits to obtain polyclonal antibodies. The terminal bleed of each rabbit recognizes the bacterial antigen and, in worm extracts, a predominant band at the expected size (18.5 kDa for SOD-1 and 24.5 kDa for SOD-2), whose intensity was drastically reduced upon specific RNAi treatment (sod-1 or sod-2 RNAi).
Western blot analysis:
After RNAi treatment, 100 young adult worms of each genotype were picked, lysed in two times loading buffer, and electrophoresed on 12% SDS–polyacrylamide gels (SDS–PAGE), and then blotted onto nitrocellulose membrane (Bio-Rad). After applying primary antibody (1:1000, rabbit polyclonal antibody against worm SOD-1 or SOD-2) and secondary antibody (1:10,000 mouse anti-rabbit IgG, Invitrogen), the membranes were incubated with the ECL plus detection reagent (Amersham Biosciences) and scanned using a Typhoon trio plus scanner. Band densities were analyzed by ImageQuant TL V2003.03.
MnSOD activity assay:
A total of 100 µl of pellets of young adult worms were ground into powder with a mortar in liquid nitrogen and mixed with 100–150 µl of extraction buffer (50 mM phosphate buffer, pH 7.8) and then were centrifuged at 4°, 10,000 x g for 10 min. The supernatant was used for electrophoresis on nondenaturing polyacrylamide gels (7.5%). After electrophoresis, the gel was soaked in 1.23 mM nitroblue tetrazolium for 10 min in the presence of 4 mM potassium cyanide to inhibit CuZn SOD activity and then soaked in 36 mM phosphate buffer (pH 7.8) containing 28 µM riboflavin and 28 mM TEMED for another 10 min. The gel was illuminated on a light box for 
10 min. The presence of the MnSOD proteins in the gel remained unstained, while the background of the gels was stained a bluish color.
O2 consumption experiment:
The rates of O2 consumption of the wild type and clk-1(qm30) and clk-1; sod-2(RNAi) and isp-1(qm150); ctb-1(qm189) were measured. Worms were grown on RNAi feeding bacteria from L3 to L4 larvae to young adult of the next generation. Young adult worms were collected and washed with M9 to be bacteria free. Oxygen consumption and protein quantification were measured as in FENG et al. (2001).
Protein damage measurement by Oxyblot:
One hundred adults from each strain or treatment or age class were picked, washed repeatedly, concentrated in 3 µl of M9 buffer, and frozen to –80° for storage. Before loading, 3 µl of lysis buffer and 4 µl 15% SDS were added into tubes. Samples were then treated by the freeze–thaw method to release protein. A total of 10 µl of DNP solution (Oxyblot) was added to the samples and kept at room temperature for 15 min, after which 7.5 µl of neutralization buffer (Oxyblot) was added. Samples (27.5 µl) were run in 7.5% SDS–PAGE gels, transferred to nitrocellulose (Bio-Rad), and blocked with 5% milk for 1 hr. Membrane was incubated with the first antibody (Oxyblot) (1:150) overnight at 4° and then for 1 hr with the second antibody (Oxyblot) (1:300) at room temperature. Membranes were incubated with the ECL plus detection reagent (Amersham Biosciences) and scanned using a Typhoon trio plus scanner. Band densities were analyzed by ImageQuant TL V2003.03. The densities of all bands in a given lane were added together and considered the Oxyblot value for the sample. Membranes were then incubated with 15% hydrogen peroxide for 30 min at room temperature and treated with a 
-tubulin antibody to derive a density value for tubulin for each lane, with which an Oxyblot value normalized to tubulin was obtained. In Figure 3, the samples for which values are represented in a given panel were always run in the same gel and treated and analyzed together to reduce experimental variations. However, sometimes more than one sample of each genotype or condition was run on the same gel, which is why the sample size is not always identical for each genotype or condition. Each membrane contained at least two samples for each strain or treatment, including the control. The relative value for each sample, including for each control sample, was obtained by dividing the value of the sample by the average value for all control samples on the same blot, which is why the control bars in Figure 3 have SEM error bars.
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Statistical analysis:
Mean post-embryonic rates were compared by using Student's t-test assuming unequal variances. Fertilities (self-brood size) were compared by using Student's t-test assuming equal variances. We performed log-rank statistics to determine if the adult life spans of the RNAi treatment groups were different from that of control groups. Embryonic viabilities and sensitivities to PQ were compared by a 
2 test (http://www.georgetown.edu/faculty/ballc/webtools/web_chi.html).
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RESULTS |
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Interactions of SOD-1 and -2 levels with each other:
The CuZn superoxide dismutase SOD-1 is expected to be expressed in the cytoplasm, but also in the mitochondrial intermembrane space (OKADO-MATSUMOTO and FRIDOVICH 2001; STURTZ et al. 2001; O'BRIEN et al. 2004). As hydrogen peroxide, which is the product of the reaction catalyzed by SODs, can cross membranes and affect cellular compartments distinct from that in which it was produced, we wondered whether the level of one type of SOD could affect the expression of the other type. We tested for this both in the wild type, as described above (Figure 1A), and in the mutants (Figure 1C). RNAi was as effective in reducing the level of the targeted SOD in the mutants as in the wild type (not shown). But we could detect no significant effects of RNAi on the levels of the untargeted SOD in the mutants or the wild type. The small effects of sod-1(RNAi) on SOD-2 levels appear minor, considering the much larger variations in SOD levels among genotypes (Figure 1B). These observations indicate that knocking down the expression of one sod gene does not induce the other and therefore suggests that superoxide detoxification is carried out independently in each cellular compartment.
Effect of sod RNAi on growth rate and other phenotypes in long-lived mutants:
We treated our collection of long-lived mutants with RNAi against sod-1 or sod-2 and examined the effect of the treatment on the length of post-embryonic development (Table 1), adult life span (Table 2), embryonic lethality (Table 3), and fertility (Table 4). In the absence of any treatment, all of the long-lived mutants display slow post-embryonic development and, except for daf-2, altered fertility. Some show increased embryonic lethality. Treatment with sod-1(RNAi) has only relatively mild effects, sometimes deleterious and sometimes slightly suppressive. For example, it appears to be deleterious for the fertility of the wild-type and daf-2 mutants, but slightly increases the fertility of clk-1 (Table 4). In fact, sod-1(RNAi) appears to be slightly suppressive for several phenotypes of clk-1 mutants, as noted previously (SHIBATA et al. 2003). However, in general, we believe that the relatively small magnitude of the effects observed here with sod-1(RNAi) precludes a clear interpretation of their meaning. On the other hand, sod-2(RNAi) appears to be clearly deleterious as it strongly slows down development of clk-1 and isp-1 (Table 1), dramatically increases embryonic lethality of these two mutants (Table 3), and decreases fertility, especially of isp-1 mutants (Table 4).
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Figure 3B shows that in the absence of any treatment only daf-2 mutants have significantly reduced levels of oxidative damage. There is also a similar tendency for clk-1 and isp-1 mutants, but the effect is minor. Figure 3, C–F, shows that sod-1 RNAi increases damage in all strains except in daf-2 mutants where, surprisingly, it tends to lower damage. Possibly, in this mutant, the excess superoxide produced by the reduction in detoxification induces an overcompensating protective mechanism involving different detoxifying enzymes. It is also of note that the reduction in damage produced by sod-1 RNAi in daf-2 mutants is consistent with the slight increase in life span produced by this treatment (Figure 4). Figure 3, C–F, also shows that sod-2 RNAi increases damage significantly in the wild type and in isp-1 mutants, but is without effect in daf-2 and clk-1 mutants. In the case of clk-1, this might be the result of a form of developmental compensation (see below). It is important to note that the effects of sod-1 RNAi on clk-1 and of sod-2 RNAi on isp-1 increase damage 1.4-fold, while there appears to be only a minor reduction in damage in untreated clk-1 and isp-1 mutants compared to the wild type (Figure 3B). Thus, the level of damage in treated mutants must be similar to, or higher than, that in the wild type.
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In the experiments described in this section of the RESULTS, for each repeat of the experiments that compared RNAi-treated and mock-treated animals (Figure 3, C–G) all the samples were analyzed on the same gels to reduce experimental variation. However, we also wanted to make sure that we could relate the relative differences between treated and untreated animals to those differences that were observed between untreated wild-type and mutant animals (Figure 3B). Thus, for this, as a control, we prepared samples from clk-1 or isp-1 mutants treated with sod-1 or sod-2 RNAi and ran them together on the same gels with samples from untreated wild-type animals (Figure 3H). We found that, in both sod-1 and sod-2 RNAi-treated isp-1 and clk-1 mutants, the level of carbonyls was indeed as high or higher as in untreated wild-type animals (Figure 3H).
In the experiments described above, we treated young animals and compared their carbonyl levels. However, it is possible that protein oxidation changes with chronological age. To test this, we chose to examine and compare carbonyl levels in the wild type and isp-1 at a specific time: the time (13 days) at which 15% of the wild-type animals but none of the mutants had died (Figure 3I). We chose isp-1 because our experiments suggest that their carbonyl levels at a young age are not significantly different from those of the wild type (Figure 3B), yet were increased by sod RNAi treatments, albeit without effects on life span (see below). Furthermore, the robust difference in life span between the wild type and isp-1 allowed for measurement that indeed compared chronological with physiological age. We found that even at the time of 15% mortality for the wild type there was no difference in carbonyl levels between the wild-type and isp-1 mutants. Thus, no spontaneous reduction in oxidative damage to proteins in the mutants can explain why isp-1 mutants survive longer than the wild type.
Effect of sod RNAi on the life span of long-lived mutants:
Although we did not find any correlation between the levels of SODs and life span in the long-lived mutant strains that we have examined (Figure 1B), the reduction of a given SOD activity should produce an increase of superoxide in the compartment where the SOD is expressed. Indeed, this must be why these reductions induce various deleterious phenotypes (Tables 1–4
) and/or reduce survival on PQ (Figure 2) and/or increase oxidative damage (Figure 3). Thus, these reductions in detoxification should shorten the life spans of these mutants if their long life spans were due to low oxidative stress. To test this hypothesis, we have examined the effects of sod RNAi on the life spans of the mutant strains, expecting to observe a total or partial suppression of their increased life spans. Surprisingly, this was not observed. Instead, we observed the following (Figure 4): (1) an almost complete absence of effect of sod-1(RNAi) except for a minor life-span-shortening effect in the wild type and an equally minor, albeit statistically significant, life-span-lengthening effect in daf-2 and (2) a life-span-lengthening effect of sod-2(RNAi) on clk-1.
The life-span-lengthening effect of sod-2(RNAi) on clk-1 is accompanied by severely increased embryonic lethality and lengthening of post-embryonic development (Tables 1–4). In addition, the treated animals are significantly smaller than the untreated animals (Figure 5A). However, they show no reduction in oxygen consumption per milligram of protein (Figure 5B) and no elevation of the amount of oxidative damage per milligram of protein (Figure 3E). One possible explanation for the apparent paradox of a decrease in ROS detoxification resulting in an increase in life span is that the sod-2 RNAi treatment of clk-1 mutants damages their respiratory chain in some way. Indeed, the phenotype of these animals is similar to that observed when wild-type worms are treated by RNAi against subunits of respiratory chain complexes (DILLIN et al. 2002; LEE et al. 2003). However, this also suggests that the increased longevity is not due to low oxidative damage, as oxidative damage is not decreased.
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Effect of sod RNAi on life span of mev-1:
In addition to testing the effect of sod RNAi on long-lived mutants, we tested the effect on the short-lived mutant mev-1 (Figure 4E). mev-1 encodes a subunit of the complex II of the respiratory chain and sustains increased oxidative stress (ISHII et al. 1998). We found that neither sod-1(RNAi) nor sod-2(RNAi) significantly altered the life span of the mutants. Yet we have shown that these treatments substantially increase oxidative stress as measured by carbonyl levels (Figure 3G). Although the short life of mev-1 mutants has sometimes been hypothesized to result from increased oxidative stress, there is no immediate demonstration of this. In fact, recent data very strongly indicate that a large part of the effect of mev-1 on life span is due to its effect on increasing programmed cell death through a direct effect on the ex pression of ced-9 (SENOO-MATSUDA et al. 2001, 2003). Our findings suggest that the degree by which sod RNAi treatment increases oxidative stress (Figure 3E) in this mutant does not shorten its life span. This is consistent with the absence of a life-span-shortening effect of sod RNAi treatments on the wild type and suggests that, as in the case of the long-lived mutants, the respiratory defect of mev-1 (SENOO-MATSUDA et al. 2001), rather than its increased oxidative stress, is the cause of its short life.
sod mRNA expression in daf-2 and clk-1 mutants:
The carbonyl levels of daf-2 and clk-1 mutants are unaffected by treatment with sod-2, although the same treatment increases carbonyl levels in the wild type. This could be due to an increase in the expression of sod-3, whose protein levels we cannot directly measure because of the absence of a SOD-3-specific antiserum. To determine whether changes in sod-3 expression might be involved in this effect, we used quantitative RT–PCR to monitor the levels of sod-2 and sod-3 mRNAs after sod-2 RNAi treatment of the wild-type and daf-2 and clk-1 mutants (Figure 6). Both sod-2 and sod-3 mRNA levels in untreated daf-2 and clk-1 mutants are elevated compared to the wild type. Treatment with sod-2 RNAi reduces sod-2 mRNA to very low levels in all three backgrounds (Figure 6A). The same treatment also reduces sod-3 levels considerably in all backgrounds. This indicates that sod-2 RNAi treatment also affects the level of sod-3, likely because of the sequence homology, which is consistent with the absence of MnSOD activity after sod-2 treatment of the wild type (Figure 1A). Note, however, that an increased level of mRNA expression does not guarantee an increase in the level of protein present, as we do not observe an elevation of SOD-2 protein in clk-1 mutants (Figure 1B). Thus, increased levels of sod-2 and -3 expression might or might not participate in the somewhat lower levels of protein carbonyls observed in daf-2 mutants (Figure 3B). However, similar increases seem to have only a minimal effect on carbonyl levels in clk-1 mutants (Figure 3B). Furthermore, sod-2 RNAi has no effect on the life span of daf-2 (Figure 4). Thus, as the total level of expression of mitochondrial sod genes is reduced by sod-2 RNAi treatment (Figures 1 and 6), the levels observed without treatment are not necessary for the long life of the mutants. The situation is similar for clk-1, where the total level of sod-2 and -3 expression in response to sod-2 RNAi treatment is lower than without treatment and thus cannot explain the absence of an increase in carbonyls in response to sod-2 RNAi. Nor, of course, can it explain why sod-2 RNAi prolongs the life span of clk-1 (Figure 4).
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and Figure 4) or as published previously (WONG et al. 1995; LAKOWSKI and HEKIMI 1996; FENG et al. 2001). Furthermore, sod(RNAi) is equally effective in reducing SOD expression to background levels in all strains (data not shown), and we have not found a correlation between the level of sod's and the sensitivity of the growth rate or of the life span to sod(RNAi).
Testing the ROS toxicity theory of aging:
From the point of view of evolutionary theory, it is a reasonable hypothesis to speculate that aging is due to the accumulation of unrepaired damage, as there is an expected trade-off between reproduction and the costs associated with somatic maintenance (HEKIMI et al. 2001b). Toxicity from the ROS produced in mitochondria is an obvious candidate for an important source of damage in this process (SOHAL 2002; DROGE 2003). In particular, aging appears to be strongly correlated with increases in oxidative stress and oxidative damage accumulation (BECKMAN and AMES 1998), in particular in the mitochondria (VAN REMMEN and RICHARDSON 2001). Yet a causal role for ROS toxicity in bringing about aging remains a fundamentally unproven hypothesis.
To test this hypothesis, we have been analyzing mutant strains of C. elegans that display an increased life span. Beyond the initial hypothesis of an involvement of ROS in life-span determination, there were numerous reasons to suppose that the phenotypes of these strains, including their life span, are due to altered ROS metabolism. One of the main sources of cellular superoxide production is believed to be the bifurcating transfer of electrons from ubiquinone to the "Rieske" iron–sulfur protein and the cytochrome b of complex III of the mitochondrial respiratory chain (RAHA and ROBINSON 2000). Two of the three long-lived mutants that we are analyzing are directly involved in this process: isp-1(qm150) is a point mutation in the iron–sulfur protein (FENG et al. 2001), and clk-1 affects the biosynthesis of ubiquinone (MIYADERA et al. 2001). The third gene, daf-2, encodes an insulin-receptor-like kinase (KIMURA et al. 1997) and affects a variety of processes in worms, including their resistance to oxidative stress (HONDA and HONDA 2002). The one short-lived mutant that we have also been studying, mev-1, is a point mutation in a subunit of mitochondrial complex II (ISHII et al. 1998).
Lack of support for the ROS toxicity theory of aging:
Strikingly, although several of the mutant strains tested have increased SOD levels (Figure 1B) and although the RNAi treatments are effective (Figure 1A), frequently appear deleterious (Tables 1–4 and Figure 2), and can be shown to increase measurable oxidative damage to proteins in the wild-type and mutant backgrounds (Figure 3), the RNAi treatments do not shorten the increased life span of the long-lived mutants (Figure 4) or worsen the short life span of mev-1 (Figure 4). Furthermore, we observe some paradoxical effects; for example, sod-1(RNAi) against daf-2 and sod-2(RNAi) against clk-1 increase life span. Thus, our results provide no substantial support for the hypothesis that the increased life span of most of the mutants that we have examined is due to low levels of ROS damage. Our results are consistent with observations using pharmacological SOD mimetics that were capable of protecting from experimental increases in oxidative stress, but were not capable of positively affecting wild-type life span in C. elegans (KEANEY et al. 2004).
Our findings about the role that oxidative stress might or might not play in the long life span of daf-2 mutants are not conclusive. Indeed, daf-2 mutants are unaffected by our treatments except in a paradoxical fashion: sod-1 RNAi decreases oxidative damage and increases life span. Thus, we have not succeeded in uncoupling low oxidative stress from the increased life span of daf-2 mutants.
A superoxide-independent role for electron transport in life-span determination:
Electron transport in the mitochondrial respiratory chain generates superoxide at sites where electrons are transferred from prosthetic groups to ubiquinone and vice versa (RAHA and ROBINSON 2000). Several of the mutants that we have examined (clk-1, isp-1, and mev-1) are closely involved in the process of electron transport (ISHII et al. 1998; FENG et al. 2001; MIYADERA et al. 2001). Thus, it is likely that these mutants have altered ROS metabolism (FENG et al. 2001; SENOO-MATSUDA et al. 2001; SHIBATA et al. 2003), as indicated by the various effects of SOD knockdowns on the mutant phenotypes of the long-lived mutants (Tables 1–4). Yet our results indicate that it is not a reduction of oxidative damage that is responsible for the observed increased life span. Our results therefore suggest that electron transport has a role in life-span determination that is independent of its role in the production of oxidative damage, as has been suggested previously on different grounds (DILLIN et al. 2002; HEKIMI and GUARENTE 2003). Our findings of the dramatic effect of sod-2(RNAi) on clk-1 (Figure 5) can also be interpreted in this light. That is, that the reduction of the activity of the mitochondrial matrix SOD-2 in the clk-1 background, with its altered quinone profile, damages the respiratory chain in a way that we do not yet understand, but that is favorable for life span. In fact, the phenotype of clk-1 mutants treated with sod-2(RNAi) is highly reminiscent of that obtained by knocking down subunits of mitochondrial complexes (DILLIN et al. 2002; LEE et al. 2003), which also prolongs life span.
Oxidative stress and aging:
The possibility that the oxidative stress theory is not generally valid has been considered by others (KOC et al. 2004; KUJOTH et al. 2005), but remains surprising (HEKIMI and GUARENTE 2003). However, most evidence about oxidative stress and aging points to a general correlation and not necessarily to a causal relationship. A scientific theory has to be falsifiable to be meaningful. Thus, although a single study cannot address at once all the observations that previously appeared to be consistent with the theory, sound experimental results that are inconsistent with a theory represent an important challenge to that theory. For example, although for technical reasons we could examine only oxidative damage to proteins, but not to other macromolecules, no part of the oxidative stress theory of aging suggests that damage to proteins does not matter in bringing about aging or suggests how an increase of oxidative stress that is sufficient to damage proteins could fail to damage other macromolecules.
More generally, it is of interest that our results—which suggest that oxidative stress from mitochondrial respiration is likely not causal in aging in the mutants that we have examined—do not imply that oxidative stress does not play a role in producing the aged phenotype in these mutants or in any other organism. In fact, a sharp increase of oxidative damage with age is well documented in many organisms and tissues. For example, oxidative damage to DNA (HAMILTON et al. 2001), measured as an increase in 8-hydroxguanosine, as well as oxidative damage to proteins (YASUDA et al. 1999; LEVINE and STADTMAN 2001), measured as an increase in carbonyls, increase dramatically with age. Interestingly, the increase in carbonyls is most marked later in life, at a time when in fact the organisms are already aged (LEVINE and STADTMAN 2001), which further implies that the increase itself cannot be causal in aging.
The higher oxidative damage of the aged phenotype suggests that aged organisms have higher level of ROS. This is supported by the finding that in Drosophila transgenic overexpression of superoxide dismutase can increase life span (SUN et al. 2002), especially in short-lived strains (ORR and SOHAL 2003). However, this again does not indicate that oxidative stress is the cause of aging but only that oxidative damage is deleterious and that relieving it in part through a genetic or pharmacological intervention might improve the health of aged organisms. In summary, while our observations suggest that oxidative stress is not the cause of aging, observations of high oxidative stress and damage in old organisms, on the other hand, are consistent with the notion that increased oxidative stress is a consequence of aging.
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0.05; **P < 0.01, #P = 0.07). The error bars are SEM. (A) Treatment of wild-type worms with paraquat (n = 5); n represents repeats of the experiment. (B) The oxidative damage levels at the young adult stage in different strains [wild type (n = 7); daf-2 (n = 8); clk-1 (n = 7); isp-1 (n = 8)]. Although there is a tendency for less damage in all three long-lived mutant strains, it is significant only in daf-2 mutants. (C) Reduction of either SOD-1 or SOD-2 levels by RNAi leads to significant increases in oxidative damage in the wild type (n = 8; no RNAi n = 7). (D) Neither sod-1 nor sod-2 RNAi significantly affects protein damage levels in daf-2 mutants (n = 8). However, there is a tendency for a reduction of damage after sod-1 RNAi treatment. (E) Reduction of SOD-1 levels but not SOD-2 levels affects oxidative damage in clk-1 mutant (n = 7). (F) Reduction of both SOD-1 and SOD-2 levels increases oxidative damage in isp-1 mutants, although only the increase produced by SOD-2 is statistically significant (n = 7). (G) Reduction of both SOD-1 and SOD-2 levels increases oxidative stress in mev-1 (n = 3 for each condition). (H) Reduction of both SOD-1 and SOD-2 levels increase carbonyls in mutants to levels that are higher than in the wild type in a direct comparison (n = 7 except for isp-1 treatment with sod-2 RNAi for which n = 5). (I) The carbonyl levels in aged isp-1 mutants (13 days old; n = 10) are not reduced compared to wild type (n = 5).
