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Genetics, Vol. 170, 1863-1877, August 2005, Copyright © 2005
doi:10.1534/genetics.105.041319
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* Department of Animal Science, University of Nebraska, Lincoln, Nebraska 68583
Department of Animal Science, North Carolina State University, Raleigh, North Carolina 27695
2 Corresponding author: Department of Animal Science, University of Nebraska, Lincoln, NE 68583-0908.
E-mail: dpomp{at}unl.edu
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ABSTRACT |
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 TOP ABSTRACT MATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSLITERATURE CITED |
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, IL6, and glucose at 8 weeks and was genotyped for 80 microsatellite markers. Since the F2 was a cross between a selection line and its unselected control, the QTL identified likely represent genes that contributed to direct and correlated responses to long-term selection for rapid growth rate. Across all traits measured, 95 QTL were identified, likely representing 19 unique regions on 13 chromosomes. Four chromosomes (2, 6, 11, and 17) harbored loci contributing disproportionately to selection response. Several QTL demonstrating differential regulation of regional adipose deposition and age-dependent regulation of growth and energy consumption were identified.
Many studies have been conducted to identify QTL for growth and body composition in mice, and several of these utilized long-term selection lines (see reviews by CORVA and MEDRANO 2001, BROCKMANN and BEVOVA 2002, and POMP et al. 2004). However, only a few of these experiments have involved crosses between a long-term selection line and its randomly selected control line (BROCKMANN et al. 1998) or between lines divergently selected from a common base population (MOODY et al. 1999; HORVAT et al. 2000). Such crosses are required to differentiate the subset of QTL that have contributed to selection response for complex traits from among those that segregate in the multitude of inbred lines used in genetical research. Recent QTL-based analyses of selection response have been reported for maize (LAURIE et al. 2004), Arabidopsis (UNGERER and RIESEBERG 2003), and Drosophila (VALENZUELA et al. 2004).
Despite the significant emphasis placed on QTL detection for growth and body composition in mice, and the fact that energy intake is a major determining factor in these phenotypes, identification of chromosomal regions harboring QTL for energy intake has proven to be elusive. SMITH RICHARDS et al. (2002) found two QTL for total intake adjusted to body weight, measured at 
10 weeks of age, on MMU17 and MMU18. MOODY et al. (1999) detected no QTL for feed intake measured from 12 to 14 weeks of age when evaluating selected chromosome regions where QTL for heat loss had been identified in a large mapping population of mice. The relative lack of information on loci contributing to genetic variation for energy consumption is a major gap in our knowledge of the control of energy balance.
This study focuses on the discovery of QTL accounting for phenotypic differences resulting from direct and correlated responses to 27 generations of selection for 3- to 6-week weight gain in mice. Selection was done within full-sib families from a base population of outbred Institute of Cancer Research (ICR) stock (EISEN 1975), resulting in the selection line designated as M16. In brief, long-term selection for rapid weight gain resulted in M16 mice that are larger than ICR mice at all ages measured (birth to 30 weeks) (EISEN 1975; EISEN et al. 1978; ROBESON et al. 1981). Correlated responses include hyperphagia with improved feed efficiency (EISEN et al. 1978; EISEN and LEATHERWOOD 1978) and increased fat, lean, and ash weights (EISEN et al. 1977). Recently, extensive recharacterization of many previously recorded phenotypes and measurement of several new phenotypes were completed using the M16 and ICR lines (ALLAN et al. 2004).
A fully inbred line (M16i) derived from M16 has been used in two previous QTL mapping experiments. A backcross population using M16i crossed with Mus musculus castaneous was measured for body weight at various ages and evaluated for body composition and skeletal development at 12 weeks. Evidence for QTL was found for all traits measured, with an extremely large effect on body weight and fatness on MMU2 (POMP 1997; LEAMY et al. 2000, 2002). A second, very large population was established using an F2 intercross between M16i and the L6 line, which was selected for low 6-week weight gain from a base population developed by crossing four standard inbred lines. Numerous QTL were detected for growth, adiposity, and reproduction with large effects also found on the distal end of MMU2 (ROCHA et al. 2004a,b,c). While both of these experiments were highly successful in QTL identification, the M16i line represents only a single sampling (family) from the M16 selection line, and it is also not possible to differentiate QTL effects originating from M16i alleles relative to alleles from the castaneous and/or L6 lines used in the crosses.
In the present study, the M16 line was crossed to its randomly selected ICR control line to create a very large F2 intercross population. By localizing QTL for a wide variety of traits related to growth and adiposity, a primary objective was to identify chromosomal regions harboring genetic variation that specifically contributed to the extensive direct and correlated phenotypic responses to 27 generations of selection for rapid weight gain. By measuring weekly feed intake during the growth phase in nearly 1200 individual F2 mice, a secondary objective was to provide sufficient power to yield a detailed map of QTL regulating energy consumption. And finally, a third objective was to begin to integrate large-scale endo-phenotyping into QTL analysis for growth and body composition to combine the powers of functional and recombination analyses (SCHADT et al. 2003; POMP et al. 2004). While the major undertaking of high-throughput evaluation of mRNA and proteomic phenotypes in the M16 x ICR F2 intercross is in progress, we demonstrate the utility of this population using obesity-relevant measurements of plasma proteins (insulin, leptin, TNF, IL6) and a metabolite (glucose).
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MATERIALS AND METHODS |
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100 generations prior to establishment of the QTL mapping population used in this study.
A large F2 population (n = 1181) was established by intercrossing the M16 and ICR lines (for a recent description of relevant phenotypes in the parental lines, see ALLAN et al. 2004). Twelve F1 families resulted from six pair matings of M16 males x ICR females and six pair matings of the reciprocal cross. A total of 55 F1 dams were mated to 11 F1 sires in sets of five F1 full sisters mated to the same F1 sire. These same specific matings were repeated in three consecutive replicates. Thus, the F2 population consisted of 55 full-sib families of up to 24 individuals each and 113/4-sib families of up to 120 individuals each. Actual numbers of mice within families varied slightly due to a small number of failed pregnancies. All litters were standardized at birth to eight pups, with approximately equal representation of males and females.
Mouse care and maintenance:
All litters were weaned at 3 weeks of age, with mice placed in plastic cages with wood chip bedding and provided ad libitum access to water and pellet feed (Teklad 8604 rodent chow). Mice were caged individually from 4 to 8 weeks of age. Laboratory temperature was maintained at 22°, with relative humidity at 35–50% and a light:dark cycle of 12:12 hr starting at 7:00 A.M. The University of Nebraska Institutional Animal Care and Use Committee approved all procedures and protocols.
Phenotypic data collection:
Body weights were measured at weekly intervals from 3 to 8 weeks of age (3WK, 4WK, 5WK, 6WK, 7WK, 8WK). From 4 to 8 weeks of age, feed intake was recorded for all F2 mice at weekly intervals (FI5, FI6, FI7, FI8). Although food spillage (any evidence of portions of the brown pellet feed present in the wood chip bedding) was marginal, data were discarded for the mice (<10%) that spilled their food. At 8 weeks of age, following a period of 1.5 hr where feed was removed but access to water remained, mice were decapitated after brief exposure to CO2. Blood was collected from the trunk, and blood glucose (GLUC) was measured using the SureStep Blood Glucose Monitoring System (LifeScan Canada, Burnaby, British Columbia, Canada). The entire body except the head (i.e., the subcranial region) was scanned in a consistent, dorsal position using a dual-energy X-ray absorption (DEXA) densitometer (PIXImus, Lunar, Madison, WI). The DEXA measurements estimated two primary body composition characters in each mouse: total subcranial tissue mass (TTM, in grams) and total subcranial fat (FAT, in grams). After scanning, each carcass was dissected and weights of liver (LIV), right hind limb subcutaneous adipose depot (SCF), and right epididymal (males) or perimetrial (females) adipose depot (EPF) were recorded. These and other tissues, including hypothalamus, pituitary, gastrocnemius muscle, heart, spleen, kidney, (with adrenal) and tails, were collected and snap frozen in LN2.
Analysis of plasma proteins:
All F2 males were measured for plasma levels of insulin (INS), leptin (LEP), tumor necrosis factor (TNF), and interleukin 6 (IL6) using a single multiplex reaction (run in duplicate) based on microsphere bead technology (Linco, St. Louis). These proteins were selected for measurement on the basis of a previous evaluation of the M16 and ICR parental lines (ALLAN et al. 2004). Assays were run according to the manufacturer's instructions using a Luminex100 system (Luminex, Austin, TX). Raw data were processed using Masterplex QT (Miraibio, Alameda, CA); plate-to-plate variation was normalized using a standard sample on all plates.
Genotyping:
DNA was extracted from tails using a protocol originally described for toe clips (POMP and MURRAY 1991). All 24 grandparents were prescreened for marker informativeness across 700 genome-wide microsatellite markers. Using the SAS program developed by ROCHA et al. (2001), markers were selected for use in the full population on the basis of maximizing informativeness in the actual F0 matings and being as evenly spaced across the genome as possible.
Genotypes were collected for 80 microsatellite markers spaced at an 20-cM average distance across 19 autosomes for all founder, F1, and F2 animals. Descriptive and map information for all markers can be found in the APPENDIX. The X chromosome was not included in the genome scan due to lack of informative markers between the parental lines (35 markers tested); we speculate that this may be due to homozygosity of large genomic regions in the ICR base population before selection of the M16 line had taken place. Genotypes were assayed using PCR with forward primers containing 19-mer 5' tails end-labeled with one of two infrared dyes (IRD700 or IRD800), followed by electrophoresis and analysis on the LI-COR 4200 DNA Analysis System (LI-COR, Lincoln, NE). Gel images were analyzed using Gene ImageIR (Scanalytics, Fairfax, VA) to determine genotypes for each individual.
Markers were evaluated for allele scoring errors on the basis of evaluation of Mendelian inheritance. All specific genotyping discrepancies were cross-referenced with the original gels and either corrected or omitted from the study. Markers were evaluated for segregation distortion in the F2 population using chi-square tests. Chromosomal linkage maps were built using Cri-Map (GREEN and CROOKS 1990) and reported in Kosambi centimorgans. Marker order was verified using the whole mouse genome sequence (http://www.ensembl.org/Mus_musculus/).
Marker positions and orders derived from the linkage analysis of the data in this study (APPENDIX) are in reasonable agreement with those from the Mouse Genome Database (MGD; http://www/informatics.jax.org) and the whole mouse genome sequence (Ensembl Genome Browser; http://www.ensembl.org/Mus_musculus/). Marker map positions estimated from the genotype data in this study were used in the subsequent QTL analyses. None of the markers used in this study deviated significantly from expected F2 Mendelian segregation ratios.
Data adjustment and analysis:
Data for LIV, EPF, and SCF were also expressed as a percentage of 8-week body weight (LIVP, EPFP, and SCFP, respectively). Percentage body fat (FATP) was defined as FAT expressed as a percentage of TTM. Body weight gain was defined as the difference between the ending weight and the starting weight for periods of 3–6 weeks (GAIN3–6) and 4–8 weeks (GAIN4–8). Weekly feed intakes were also adjusted for the body weight measured at the end of each weekly period (FI5A, FI6A, FI7A, FI8A). Weekly feed efficiency was defined as weight gain divided by total feed intake for each weekly period (FE5, FE6, FE7, FE8; e.g., FE5 = (5WK–4WK)/FI5). Total adjusted feed intake (FIA) and feed efficiency (FE) were calculated over the entire 4-week feeding period.
Basic statistics and trait distributions were calculated using the UNIVARIATE function in SAS (SAS 1990). Phenotypic correlations among dependent variables were adjusted for sex and replicate using the MANOVA procedures in SAS (SAS 1990). Correlations are reported for 16 of the 29 traits used in QTL analysis; traits selected for this analysis represented four primary categories of phenotypes, including growth, body composition, energy consumption, and blood metabolites.
Data for body weights, feed intakes, organ weights, body composition traits, and blood glucose were analyzed with a mixed model approach using the PROC MIXED procedures in SAS (LITTLE et al. 1996). The model contained sire and dam nested within sire as random effects, replicate and sex as fixed effects, and all two-way interactions. Normalized data for insulin, leptin, TNF, and IL6 were analyzed with no sex effect (or corresponding interactions) in the model.
Residuals generated from the mixed model for each trait were combined with marker genotypes and map information for discovery of QTL using the F2 regression analysis option of QTL Express (SEATON et al. 2002). The analysis involved three steps. First, a simple interval approach, using a single QTL model, was run. This step was followed by selection of QTL to be used as background genetic effects to increase the precision and accuracy of QTL discovered in the single QTL model, which is similar in nature to a composite interval mapping analysis (ZENG 1993, 1994). Selection of QTL for genetic background effects was done using a forward selection approach. Briefly, the QTL with the largest effect was added to the model as a cofactor and the analysis rerun. This procedure was repeated until no additional significant QTL were detected. The final step was to remove each QTL individually from the model and rerun the analysis with the background genetic effects, as suggested by ZENG (1993).
Analysis for two QTL in a region was done (QTL Express) for all QTL with large confidence intervals. Results of all such analyses were not significant (data not shown). To test for QTL x sex interaction effects, data were reanalyzed without preadjustment for gender and by fitting a QTL x sex interaction into the model. To evaluate whether QTL were fixed or still segregating in the parental lines, analyses were also performed within the 11-sib families for several regions where QTL were identified in the full population.
A previous study using the M16i line (ROCHA et al. 2004a) showed that basing QTL analyses on residuals from models that preadjust data introduced a consistent 10–20% downward bias in estimates of most QTL effects. However, no such bias was found in this study; QTL effects (and LOD scores) were essentially unchanged between analyses based on residuals and those where effects were fitted within the QTL analysis itself (data not shown). We speculate that the lack of bias in the present analysis is due to the large and well-balanced sibships created in this F2 population structure.
The percentage variance explained by a QTL effect was calculated as follows: [(residual variance of the reduced model – residual variance of the full model)/residual variance of the reduced model] x 100. Confidence intervals were calculated for the position of the QTL using a bootstrapping resampling option (VISSCHER et al. 1996) in QTL Express (SEATON et al. 2002), with 1000 iterations for each chromosome.
Genome-wide significance thresholds from the regression analysis were established using permutation testing (CHURCHILL and DOERGE 1994). A total of 1000 permutations were conducted for a variety of traits, including 8WK, FATP, LIVP, INS, FE, and FIA. Due to the similarity of thresholds for all of these six traits, and for computational simplicity, we used their average value (LOD 3.3) to establish a standard 5% genome-wide significance threshold for all traits in the study. The same approach was used in previous studies (ROCHA et al. 2004a,b) that involved a similarly large F2 population originating from a cross involving the M16i line. Since relatively limited QTL data exist for feed intake and for plasma levels of insulin, leptin, TNF, and IL6, a 10% genome-wide significance level (LOD 2.9) was also used as suggestive evidence for QTL for these traits.
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RESULTS |
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Figure 1 summarizes the genomic mapping of direct and correlated responses to long-term selection for 3- to 6-week weight gain in mice, representing all QTL identified in the M16 x ICR F2 cross. The most extensive contributions to selection response were made by QTL on chromosomes 2, 6, 11, and 17, which yielded 72% of the 95 total QTL found in this study and 42% of the estimated 19 independent chromosomal regions harboring unique QTL. Moreover, these four chromosomes harbored all of the QTL found, representing a direct response to selection for 3- to 6-week weight gain. Extensive pleiotropy more than likely exists across the gamut of traits measured, although, on the basis of the locations of QTL peaks and confidence intervals, several chromosomal regions appear to harbor multiple QTL (Figures 4 and 5).
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DISCUSSION |
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Growth and body composition:
Many of the locations of QTL for growth and body composition traits found in this M16 x ICR F2 intercross coincide with QTL positions from previous studies using the M16i line (LEAMY et al. 2002; ROCHA et al. 2004a,b) or a variety of other mouse crosses (see POMP et al. 2004 and SNYDER et al. 2004 for summaries). Relative to the earlier studies using the M16i line (a fully inbred line derived from an M16 full-sib mating), the present results are able to inform us regarding which previously identified QTL have made the largest contributions to selection response in M16. Because the majority of these growth and body composition QTL have already been assigned locus symbols (see http://www.informatics.jax.org/searches/marker_forms.html), we have assigned new symbols only to the loci detected in this study for traits related to energy consumption and hormone/metabolite levels. Once QTL are resolved at the gene level, the many symbols assigned to specific regions for similar or correlated traits can be coalesced and reduced as necessary.
It is interesting that ROCHA et al. (2004a) found several more regions harboring QTL for growth traits in addition to what was detected in this study. Although the experiments had relatively similar power of detection in terms of informative meioses, there was greater phenotypic divergence in the M16i x L6 cross employed by ROCHA et al. (2004a). While the L6 line was selected for a low 6-week body weight, the ICR line used as the base population for M16 originated from stock selected for fecundity and size (HAUSCHKA and MIRAND 1973). Furthermore, M16i represents a fully inbred line while M16 likely still segregates alleles at some QTL, although a more thorough evaluation of this will require denser genotyping (see RESULTS). Thus, several explanations exist for the discrepancy in detected QTL. First, some QTL found in the M16i x L6 cross may be present in the M16 x ICR cross but were not detected due to either smaller phenotypic divergence or ongoing segregation within M16. Such undetected QTL would have relevance to understanding the selection response in the M16 line and may help indicate why a relatively small proportion of the variation in the traits measured in the M16 x ICR cross was explained by the detected QTL. Second, and possibly unrelated to selection response in M16, QTL found previously but not identified in the current cross may represent the effects of alleles contributed by the L6 line.
QTL and selection response:
In mice, HORVAT et al. (2000) performed a genome-wide QTL analysis using the high-fat (F) and low-fat (L) lines that had been divergently selected for 53 generations on the basis of the percentage of body fat. As in the present study, HORVAT et al. (2000) also found evidence for four primary regions that contributed to long-term selection response, although the effects of the QTL that they detected were in general larger than those quantified in the M16 x ICR cross. This is likely a result of the use of divergent selection lines, as opposed to a selection line and its control, and hence of greater phenotypic divergence. Also, nearly twice as many generations of selection had taken place in the F and L lines relative to M16. Interestingly, none of the four regions contributing to selection response in this study and in that of HORVAT et al. (2000) appear to overlap, although it should be noted that we did not consider MMUX in the current analysis. In an experiment of very similar nature (but smaller magnitude) to what we report here, BROCKMANN et al. (1998) searched for QTL influencing body weight and fatness in crosses between a high-body-weight selection line (DU6) and its unselected control line (DUKs). Significant QTL were found for body weight on MMU11 (in relatively close proximity to that found in this study); for abdominal fat weight on MMU4, MMU11, and MMU13; and for abdominal fat percentage on MMU3 and MMU4. Together, the detected QTL contributed about one-third of the phenotypic variance of body weight and abdominal fat weight in the F2 population.
Cumulatively, these experiments using QTL analysis to map genomic regions contributing to long-term selection response for growth and fatness in mice lead to several putative conclusions. First, selection from different base populations appears to operate, for the most part, on genetic variation located in different regions of the genome. This is interesting in that most long-term selection experiments for growth- and/or fat-related traits seem to lead to very similar phenotypic consequences (EISEN 1989). Second, although several QTL with significant effects can be localized when crossing divergently selected lines or a selection line and its control, a significant portion of selection response remains undetected at the genomic level. In a very large evaluation of the genetic architecture of response to very long-term selection for oil concentration in the maize kernel, LAURIE et al. (2004) found evidence for >50 QTL combining to account for 50% of the genetic variance. They attributed the fact that not all the variation could be accounted for to several factors, including potential underestimation of QTL effects, confounding epistatic interactions, and additional QTL that remained undetected in their experiment. In support of the latter argument, ROCHA et al. (2004a) concluded that while QTL effects for body weight in mice clearly do not conform with the uniform distribution proposed in the context of an infinitesimal model, they approximate an exponential model that "nonetheless maintains an infinitesimal quality."
Although selection for 3- to 6-week weight gain was originally replicated (EISEN 1975), the replicates were crossed to form the existing single lines of M16 and ICR, and thus there is no mechanism to differentiate QTL representing selection response from those that may have arisen from random genetic drift. While the strong phenotypic changes originally observed in the M16 line shortly after selection was completed have been remarkably resilient even after 100 generations of relaxed selection (ALLAN et al. 2004), and while nearly all effects of M16 alleles at QTL found in this study were in the expected direction, it is still likely that genetic drift has had significant impact on gene frequency and genetic variance in M16 (see WALSH 2004). This may explain some of the genomic regions harboring QTL for a variety of correlated traits but lacking a QTL for 3- to 6-week weight gain. Alternatively, such regions may still represent direct responses to selection, but the experiment contained sufficient power to detect QTL for the correlated traits only.
While the QTL detected in this study are most likely the result of selection acting on genetic variation present in the original ICR base population, they may also have originated from new mutations that took place during selection (see KEIGHTLEY 2004). Although new mutations influencing growth may also have arisen during the extended period of relaxed selection, there was no selection pressure to propagate such alleles. And since such mutations would have been equally likely in M16 and ICR, QTL with alleles of ICR origin that increase body weight would have been observed. Two examples of such QTL were found in this study.
Given that the QTL detected in this study for growth and fatness have, for the most part, been identified in previous crosses using M16i and given that an extensive comparison and contrasting of these with many other QTL reports was provided by ROCHA et al. (2004a)(b; see also Figure 1 in POMP et al. 2004), we will not repeat that endeavor here. In brief, MMU2 had significant QTL for almost all measured traits related to growth and body composition. The extreme contribution of regions of MMU2 derived from the M16 line to the biology of fat deposition and growth have been summarized recently by JEREZ-TIMAURE et al. (2004)(2005). Multiple QTL appear to exist on MMU6, in agreement with several other reports (CHEVERUD et al. 2001; MASINDE et al. 2002; ROCHA et al. 2004a). The QTL with strongest effects were localized to MMU11. Previous studies have shown evidence for clustering of genes with variable gene expression to MMU11 in the same locations as QTL from this study. This supports the idea of having variation within multiple genes contribute to the effects localized under a single QTL peak (DE HAAN et al. 2002; JEREZ-TIMAURE et al. 2005). The proximal half of MMU17 has been shown to include a number of QTL for traits related to fat and growth, as was verified in this study.
QTL and tissue-specific regulation:
Evidence for QTL with depot-specific regulation of fat mass, as found in the M16 x ICR cross on MMU4 and MMU7, is important when trying to understand the polygenic nature of adiposity in mammals. Microarray studies in rats comparing mRNA from visceral and subcutaneous fat found gene expression to be differentially regulated between the fat pads (ATZMON et al. 2002). Many of the genes found to be up- and downregulated were predominantly involved in glucose homeostasis, insulin action, and lipid metabolism. In humans, regional depot differences have been observed for gene expression, insulin sensitivity, and fatty acid metabolism (MONTAGUE et al. 1998; VIDAL 2001; WAJCHENBERG et al. 2002). Others have mapped QTL for depot-specific fat deposition to the same regions of MMU4 (MOODY et al. 1999) and MMU7 (KEIGHTLEY et al. 1998; TAYLOR et al. 2001; ROCHA et al. 2004b). JEREZ-TIMAURE et al. (2005), in their fine mapping of QTL on MMU2, found a QTL for epididymal fat as a percentage of body weight with no effect on total percentage of body fat. MEHRABIAN et al. (1998) found similar results for MMU2 showing QTL contributing to specific fat depots.
QTL and age-specific regulation:
Examples of age-dependent QTL effects for body weight have been shown in many studies (e.g., BROCKMANN et al. 2004; ROCHA et al. 2004a). In this study, growth was evaluated only from 3 to 8 weeks of age. Yet, we still see genetic regulation that is period specific within this narrow window of observation, which can be interpreted as evidence for different subsets of genes contributing to growth as ontogeny progresses. Results showing differences in cell number and cell size at different ages have been observed in several selection experiments in mice (FALCONER et al. 1978; ATCHLEY et al. 2000), including the M16 line (EISEN and LEATHERWOOD 1978).
QTL for energy consumption:
A major objective of this study was to uncover evidence for QTL regulating energy consumption in mammals, a goal that has proven elusive in past studies. Greater success has been achieved in studies using birds. VAN KAAM et al. (1999) found one QTL within a fixed-age interval that showed significant linkage for feed intake. DE KONING et al. (2003) reported a QTL for residual feed intake on chromosome 4 in broiler lines. In mice, MOODY et al. (1999) failed to detect QTL for feed intake in specific chromosomal regions harboring QTL for heat loss in a large mapping population (n = 560), where consumption was measured over a 14-day period beginning at 12 weeks of age in mice. More recently, SMITH RICHARDS et al. (2002) found QTL for macronutrient diet intake in mice with two QTL for total intake adjusted to body weight on MMU17 and MMU18; intake was measured over a 10-day period in mice ranging from 9 to 11 weeks of age.
In this study, we report significant evidence for a total of 12 QTL affecting measures of energy intake and efficiency of growth relative to feed consumption. Analysis of total intake adjusted for body weight (FIA) yielded five significant QTL, with the largest effect found on MMU11 explaining 4.7% of the residual variance. The success achieved using the M16 x ICR cross may be attributed to one or more of several possible explanations. Notably, this study used the largest sample size yet to be employed in the search for QTL regulating energy consumption. Perhaps more importantly, feed intake was measured at younger ages than in the past, specifically targeting periods of rapid growth as opposed to time points corresponding more to maintenance of body weight. This may also explain why higher correlations between feed intake and body weight were observed in this experiment relative to those reported by SMITH RICHARDS et al. (2002). The three QTL found in this study for intake at specific weekly intervals show, for the first time, age-dependent genomic regulation of feed intake and feed efficiency in a fashion similar to what has been widely observed for body weight.
QTL for endo-phenotypes:
A primary motivation for establishing this very large M16 x ICR F2 intercross population was to begin to integrate large-scale endo-phenotyping into QTL analysis for growth and body composition to combine the powers of functional and recombination analyses (e.g., SCHADT et al. 2003; POMP et al. 2004). Evaluation of segregating populations at the transcriptional and proteomic levels will greatly facilitate a more thorough understanding of response to selection for rapid growth rate, and the overall genetic architecture of complex traits such as body weight and adiposity. To this end, we collected and stored a large number of tissues from each of the nearly 1200 F2 mice, including hypothalamus, pituitary, liver, skeletal muscle, epididymal/perimetrial adipose, subcutaneous adipose, kidney, and blood. While the major undertaking of high-throughput evaluation of mRNA and proteomic phenotypes in the M16 x ICR F2 intercross is in progress using several of these tissues, we measured in this study the levels of several plasma proteins (insulin, leptin, TNF, IL6) and a metabolite (glucose) relevant to growth and obesity.
Previously, we showed that M16 male mice have fasted blood glucose levels that classify them as type II diabetic (ALLAN et al. 2004). While only two QTL for blood glucose levels were found in this study, similar QTL have been previously reported in two mapping populations using mouse models for type II diabetes (HIRAYAMA et al. 1999; UEDA et al. 1999). A possible explanation for finding only two significant QTL for blood glucose in the M16 x ICR F2 intercross may be related to the relatively brief (1.5-hr) fasting period used prior to glucose measurements. More importantly, strong interaction effects were detected between QTL for blood glucose levels and gender.
The QTL detected on MMU2 for insulin in this study are in agreement with previous reports (MEHRABIAN et al. 1998; HIRAYAMA et al. 1999), while a study mapping QTL in recombinant inbred lines (SMXA; ANUNCIADO et al. 2003) found a suggestive QTL for insulin levels in females on MMU17 that may correspond to what was found in the M16 x ICR cross. The suggestive evidence that we found for an insulin QTL on MMU11, proximal to the QTL seen for growth, fat, and glucose levels, supports a previous report by LEITER et al. (1998). For leptin levels, two QTL were identified in the same regions as those found for insulin. Colocalization of QTL for insulin and leptin levels would not be surprising, given the positive correlations between these traits. ROBINSON et al. (2000) have shown that most hyperinsulinemic (insulin-resistant) mouse models are also hyperleptinemic (leptin resistant).
All of the QTL found for insulin and leptin levels were trans in nature (i.e., the QTL regulating variation in levels of each protein are distinct from the structural loci coding for each protein). We were unable to detect QTL for TNF and IL6. Differences in the M16 and ICR parental lines for levels of these proteins were significant but not large (ALLAN et al. 2004). The substantial standard error associated with measurement of these proteins, coupled with an apparent lack of correlation to growth and fat phenotypes in the M16 x ICR cross, likely explains our inability to detect QTL for these proteins. We are not aware of any findings of QTL for plasma levels of either TNF or IL6 in mice in other experiments as well.
Summary:
By mapping QTL in a large F2 population resulting from an intercross between the M16 and ICR lines of mice, we have identified chromosomal regions harboring genes that likely contributed to direct and correlated responses to long-term selection for rapid growth rate in mice. The evaluation of QTL for food consumption represents the most extensive and successful analysis conducted to date of polygenic control of energy intake in a growing animal, although this still comprises a relatively poor understanding of this complex trait. We are currently conducting very dense genotyping and evaluating global gene expression across multiple tissues in this F2 population. Such additional data not only will provide significantly strengthened power to understand the nature and mechanisms of selection response for growth, but also will assist in identifying and prioritizing candidate genes underlying QTL for body weight, fatness, and traits related to energy balance.
APPENDIX Microsatellite markers genotyped in the M16 x ICR F2 population
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a Positions of markers in Kosambi centimorgans. First centimorgan column is from Cri-Map analysis; the second centimorgan column is from the MGD.
b Marker informativeness as evaluated by QTL Express.
c Positions of the first marker on each chromosome are from the MGD and thus will be the same for both columns.
d Estimates are based on Ensembl map positions of genes shown to be tightly linked in the MGD.
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ACKNOWLEDGEMENTS |
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FOOTNOTES |
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TOPABSTRACTMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSLITERATURE CITED |
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ALLAN, M. F., E. J. EISEN and D. POMP, 2004 The M16 mouse: an outbred animal model of early onset polygenic obesity-induced diabetes (diabesity). Obes. Res. 12: 1397–1407.[Medline]
ANUNCIADO, R. V., M. NISHIMURA, M. MORI, A. ISHIKAWA, S. TANAKA et al., 2003 Quantitative trait locus analysis of serum insulin, triglyceride, total cholesterol and phospholipid levels in the (SM/J x A/J) mice. Exp. Anim. 52: 37–42.[CrossRef][Medline]
ATCHLEY, W. R., R. WEI and P. CRENSHAW, 2000 Cellular consequences in the brain and liver of age-specific selection for rate of development. Genetics 155: 1347–1357.
ATZMON, G., X. M. YANG, R. MUZUMDAR, X. H. MA, I. GABRIELY et al., 2002 Differential gene expression between visceral and subcutaneous fat depots. Horm. Metab. Res. 34: 622–628.[CrossRef][Medline]
BROCKMANN, G. A., and M. R. BEVOVA, 2002 Using mouse models to dissect the genetics of obesity. Trends Genet. 18: 367–376.[CrossRef][Medline]
BROCKMANN, G. A., C. S. HALEY, U. RENNE, S. A. KNOTT and M. SCHWERIN, 1998 Quantitative trait loci affecting body weight and fatness from a mouse line selected for extreme high growth. Genetics 150: 369–381.
BROCKMANN, G. A., E. KARATAYLI, C. S. HALEY, U. RENNE, O. J. ROTTMANN et al., 2004 QTLs for pre- and postweaning body weight and body composition in selected mice. Mamm. Genome 15: 593–609.[CrossRef][Medline]
BUNGER, L., A. LAIDLAW, G. BULFIELD, E. J. EISEN, J. F. MEDRANO et al., 2001 Inbred lines of mice derived from long-term divergent selection on fat content and body weight. Mamm. Genome 12: 678–686.[Medline]
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