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Feeding states solely modulated thermotaxis behavior. (A and D) Experimental procedure for feeding-state-shifted assay at the same cultivation temperature. An ellipsoid indicates conditioning plate: a solid ellipsoid indicates cultivation with food and an open ellipsoid indicates cultivation without food. (B) Well-fed animals were transferred to conditioning plate without food at 17°. About 20 animals were examined at each time point in six trials. Animals food deprived for 1 hr had significant differences from naive animals (P < 0.01), and animals starved for >1.5 hr had significant differences (P < 0.0001). (C) Well-fed animals were transferred to conditioning plate without food at 25°. About 10 animals were examined at each time point in seven trials. Animals food deprived for >10 min had significant differences from naive animals (P < 0.0001). (E and F) Starved animals were recultivated with food at the same cultivation temperature (17° or 25°). About 10 animals were examined at each time point in 10 (17°) and 7 (25°) trials. Animals recultivated with food for >10 min had significant differences from starved animals (P < 0.0001). (B, C, E, and F) Thermotaxis was evaluated using "fraction of 17," which includes the class "17" and "fraction of 25," which includes the class "25." A square indicates fraction of 17 and a circle indicates fraction of 25. Top arrows indicate cultivation temperature and feeding state; "f" indicates cultivation with food and "s" cultivation under food-deprived conditions. Error bar indicates SD.
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