Changes in the Localization of the Saccharomyces cerevisiae Anaphase-Promoting Complex Upon Microtubule Depolymerization and Spindle Checkpoint Activation
Genetics Melloy and Holloway
167: 1079
Supplement to Melloy et al. 2004
Figure S1
Files in this Data Supplement:
Figure S1
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Figure S1: Spindle microtubules in the okp1-5 appear normal yet longer at non-permissive temperature.
Shown are okp1-5 cells grown at 23° or 37° for four hours then fixed for indirect immunofluorescence using a rat anti-a-tubulin YOL1/34 primary antibody and a FITC anti-rat secondary antibody. FITC (a,b) and corresponding DIC (c,d) images are shown. The percent of cells with a normal spindle length are shown below each category. Arrowheads in (b) highlight longer spindle length found in many okp1-5 mutant cells [compare to arrowheads in (a)].
