Figure 2.
The eat-20::gfp fusion gene and eat-20 RNA expression in wild-type animals (A–E). The anterior side is to the left and the dorsal side is up. Bars, 10 µm. (A) In the head region of an adult, GFP is expressed in the pharynx (asterisk) and in IL1 and OLQ neurons. (B) The same animal as in A at a different focal plane. BAG, IL1, and OLQ neurons and the pharynx express GFP. (C) In the tail region of an adult, GFP is expressed in the left ALN neuron (arrow) and the right ALN neuron (arrowhead), which is out of the focal plane. The outline of the animal is indicated by a broken line. (D) GFP fluorescence is observed in the procorpus, metacorpus, and terminal bulb of an adult pharynx. (E) GFP fluorescence is observed in I4 at L1. A broken line indicates the pharynx. (F) A 1.5-fold stage embryo. eat-20 RNA is expressed in the presumptive pharynx (arrowheads) and the intestine (arrows). (G) A threefold stage embryo. eat-20 RNA is expressed in the pharynx. (H) eat-20 RNA is expressed in the pharynx, the pharyngeal-intestinal valve (arrows), and some unidentified cells around the pharynx in an adult.
