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Corrigendum
for
Bierne et al., Genetics 148 (4) 1893-1906.
Genetics, Vol. 150, 1331-1331, November 1998, Copyright © 1998
= 0.05) for the..."):
CORRIGENDA
In the article by N. BIERNE, S. LAUNEY, Y. NACIRI-GRAVEN and F. BONHOMME (Genetics 148: 18931906, April 1998) entitled "Early effect of inbreeding as revealed by microsatellite analyses on Ostrea edulis larvae" the following text is inserted at the bottom of page 1896 (preceded by, "...and significant at the single-test level (
= 0.05) for the..."):
remaining two loci (Oedu.T5 and Oedu.B0) at day 70. No temporal heterogeneity between stages was observed (Gh, P > 0.05) and data were therefore pooled. On pooled data, heterozygote excesses are significant at the experimentwise level (= 0.0125) for thee loci (Oedu.O9, Oedu.T5, and Oedu.J12) and at the single test level for the fourth locus (Oedu.B0, P = 0.0246). The comparison of observed vs. expected MLH distributions corroborates the single-locus observations (Figure 1). An excess of higher multiheterozygous genotypes is observed, which is not significant at day 10 (G = 6.213, d.f. = 3, P = 0.10; without Oedu.J12: G = 5.987, d.f. = 2, P = 0.11; without Oedu.O9: G = 3.97, d.f. = 2, P = 0.26) but becomes significant at day 70 (G = 29.206, d.f. = 3, P = 2 x 10-6; without OeduJ12: G = 19.68, d.f. = 2, P = 2 x 10-4; without Oedu.O9: G = 20.4, d.f. = 2, P = 10-5). No significant temporal heterogeneity was detected at the single locus level, whereas all loci exhibited an increase of the heterozygote excess D (Table 3). At the multilocus level, however, a significant temporal heterogeneity was found between MLH distributions at days 10 and 70 (Gh = 8.534, d.f. = 3, P = 0.036).
Cross C2: No significant heterozygote excess was observed at the youngest stage (day 1, Table 3). At days 10 and 70, Oedu.O9 exhibited significant heterozygote excesses at the experimentwise level (= 0.0056), whereas D was significant only at the single-test level for Oedu.B0 (P = 0.0068 and P = 0.0246). No significant temporal heterogeneity was found for Oedu.O9 (Gh = 2.288, P > 0.05), and the Gp test exhibited a highly significant heterozygote excess on pooled data (Gp = 16.457, P = 5.1 x 10-5). For Oedu.B0, a significant heterogeneity of heterozygote frequencies across stages was detected (Gh = 9.903, P = 0.0071). Because one may postulate that heterogeneity is due to homozygote mortality during the larval phase, partial heterogeneity tests were performed to determine which transition accounts for this. A temporal heterogeneity was detected between days 1 and 10 (Gh = 7.254, d.f. = 1, P = 0.0071) but not between days 10 and 70 (Gh = 0.108, d.f. = 1, P = 0.74). However, days 1070 pooled data showed a highly significant heterozygote excess (Gp = 12.257, d.f. = 1, P = 0.0005). Figure 1 shows that excesses of multiheterozygous genotypes were already present at the end of the larval stage (day 10: G = 12.189, d.f. = 2, P = 0.0023,
= 0.0167) and later (day 70: G = 8.159, d.f. = 2, P = 0.017,
= 0.0167) but were not detected at the beginning of the free larval stage (day 1), for which no deviation from the expected MLH distribution is observed (G = 0.85, d.f. = 2, P = 0.6538.) No temporal heterogeneity was detected (Gh = 5.148, d.f. = 4, P = 0.27) using the three loci. Removing Oedu.T5, which perhaps behaved neutrally because homozygotes are not autozygotes, we obtained, however, a significant heterogeneity between days 1 and 10 two-locus MLH distributions (Gh = 8.888, concluding with, "d.f. = 2, P = 0.012) but not between days 10 and 70 (Gh = 0.718, d.f. = 2, P = 0.6983)."
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