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doi:10.1534/genetics.109.105213
A more recent version of this article appeared on September 1, 2009.
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Recombinase-Mediated Cassette Exchange Provides a Versatile Platform for Gene Targeting: Knockout of miR-31b
Ruifen Weng 1, Ya-Wen Chen 1, Natascha Bushati 1, Adam Cliffe 1 and Stephen M. Cohen 1*
1 Temasek Life Sciences Laboratory
* To whom correspondence should be addressed. E-mail: cohen{at}tll.org.sg.
Submitted on May 19, 2009
Accepted on 16 June 2009
A series of vectors have been designed to enhance the versatility of targeted homologous recombination, as a means to make user-defined mutations in Drosophila melanogaster. Recombinase mediated cassette exchange greatly improves flexibility, permitting sequential targeting at any locus. Application of RMCE to delete an intronic microRNA gene is described.
Key Words: gene knock-out, homologous recombination, microRNA
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