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doi:10.1534/genetics.108.094508
A more recent version of this article appeared on November 1, 2008.
NOTE |
A Simple PCR-based Method for the Construction of RMCE Donor Vectors
Jack R. Bateman 1* and Chao-ting Wu 2
1 Bowdoin College
2 Harvard Medical School
* To whom correspondence should be addressed. E-mail: jbateman{at}bowdoin.edu.
Submitted on July 29, 2008
Revised on September 3, 2008
Accepted on 4 September 2008
Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase mediated cassette exchange (RMCE) using the 
C31 integrase. This PCR-based strategy employs small attB 'tails' on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.
Key Words: Drosophila, RMCE, attB40, phiC31, targeted transgenesis