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doi:10.1534/genetics.105.041616
A more recent version of this article appeared on July 1, 2005.
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Centric regions of soybean (Glycine max L. Merr.) chromosomes consist of retroelements and tandemly repeated DNA and are structurally and evolutionarily labile
Jer-Young Lin 1, Barbara Hass-Jacobus 1, Phillip SanMiguel 1, Jason G Walling 1, Yinan Yuan 1, Randy C Shoemaker 2, Nevin D Young 3 and Scott Jackson 1*
1 Purdue University
2 USDA-ARS-CICGR, and Department of Agronomy
3 University of Minnesota
* To whom correspondence should be addressed. E-mail: sjackson{at}purdue.edu.
Submitted on February 3, 2005
Revised on March 16, 2005
Accepted on 31 March 2005
Little is known about the physical makeup of heterochromatin in the soybean (Glycine max L. Merr.) genome. Using DNA sequencing and molecular cytogenetics, an initial analysis of the repetitive fraction of the soybean genome is presented. BAC 076J21, derived from linkage group L, has sequences conserved in the pericentromeric heterochromatin of all 20 chromosomes. FISH analysis of this BAC and three subclones on pachytene chromosomes revealed relatively strict partitioning of the heterochromatic and euchromatic regions. Sequence analysis showed that this BAC consists primarily of repetitive sequences such as a 102 bp tandem repeat with sequence identity to a previously characterized ~120 bp repeat (STR120). Fragments of Calypso-like retroelements, a recently inserted SIRE1 element and a SIRE1 solo-LTR were present within this BAC. Some of these sequences are methylated and are not conserved outside of G. max and G. soja, a close relative of soybean, except for STR102 that hybridized to a restriction fragment from G. latifolia. These data present a picture of the repetitive fraction of the soybean genome that is highly concentrated in the pericentromeric regions, consisting of rapidly evolving tandem repeats with interspersed retroelements.
Key Words: BAC, FISH, heterochromatin, repetitive DNA, soybean
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