LINKAGE OF MUTATIONS AFFECTING MINUS FLAGELLAR MEMBRANE AGGLUTINABILITY TO THE mt^- MATING-TYPE LOCUS OF CHLAMYDOMONAS

Carol J. Hwang ¹, Brian C. Monk ¹, and Ursula W. Goodenough ¹

¹ Department of Biology, Washington University, St. Louis, Missouri 63130

Two independently isolated mutant strains, imp-10 and imp-12, were obtained by UV irradiation of wild-type mating-type minus (wt^-). Each fails to agglutinate sexually with gametes of either mating type, but mating and zygote formation can be elicited by agglutinating either strain to wt⁺ gametes by means of anti-flagellar antiserum. Tetrad analysis of the resultant zygotes shows that both imp-10 and imp-12 are very closely linked to mt^-, with no recombinants observed. Diploid strains constructed between imp-10 or imp-12 and wt⁺ gametes are wt^-, that is, they agglutinate and fuse like normal minus cells. Tetrad analysis of triploids from imp-10 diploid x wt⁺ haploid crosses shows that only imp-10 and wt⁺ products are recovered. A model is proposed to account for these results.

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