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AN ACETYLCHOLINESTERASE-DEFICIENT MUTANT OF THE NEMATODE CAENORHABDITIS ELEGANS
Carl D. Johnson 1, John G. Duckett 1, Joseph G. Culotti 1, Robert K. Herman 2, Philip M. Meneely 2, and Richard L. Russell 1
1 Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260
2 Department of Genetics and Cell Biology, University of Minnesota, St. Paul, MN 55101
Within a set of five separable molecular forms of acetylcholinesterase found in the nematode Caenorhabditis elegans, previously reported differences in kinetic properties identify two classes, A and B, likely to be under separate genetic control. Using differences between these classes in sensitivity to inactivation by sodium deoxycholate, a screening procedure was devised to search for mutants affected only in class A forms. Among 171 previously isolated behavioral and morphological mutant strains examined by this procedure, one (PR946) proved to be of the expected type, exhibiting a selective deficiency of class A acetylcholinesterase forms. Although originally isolated because of its uncoordinated behavior, this strain was subsequently shown to harbor mutations in two genes; one in the previously identified gene unc-3, accounting for its behavior, and one in a newly identified gene, ace-1, accounting for its selective acetylcholinesterase deficiency. Derivatives homozygous only for the ace-1 mutation also lacked class A acetylcholinesterase forms, but were behaviorally and developmentally indistinguishable from wild type. The gene ace-1 has been mapped near the right end of the X chromosome. Gene dosage experiments suggest that it may be a structural gene for a component of class A acetylcholinesterase forms.
Submitted on July 21, 1980Revised on January 19, 1981
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