- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Chattoo, B. B.
- Articles by Sherman, F.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Chattoo, B. B.
- Articles by Sherman, F.
PATTERNS OF GENETIC AND PHENOTYPIC SUPPRESSION OF lys2 MUTATIONS IN THE YEAST SACCHAROMYCES CEREVISIAE
Bharat B. Chattoo 1, Edward Palmer 1, Bun-Ichiro Ono 1, and Fred Sherman 1
1 Department of Radiation Biology and Biophysics University of Rochester School of Medicine and Dentistry Rochester, New York 14642
A total of 358 lys2 mutants of Saccharomyces cerevisiae have been characterized for suppressibility by the following suppressors: UAA and UAG suppressors that insert tyrosine, serine or leucine; a putative UGA suppressor; an omnipotent suppressor SUP46; and a frameshift suppressor SUF1–1. In addition, the lys2 mutants were examined for phenotypic suppression by the aminoglycoside antibiotic paromomycin, for osmotic remediability and for temperature sensitivity. The mutants exhibited over 50 different patterns of suppression and most of the nonsense mutants appeared similar to nonsense mutants previously described. A total of 24% were suppressible by one or more of the UAA suppressors, 4% were suppressible by one or more of the UAG suppressors, while only one was suppressible by the UGA suppressor and only one was weakly suppressible by the frameshift suppressor. One mutant responded to both UAA and UAG suppressors, indicating that UAA or UAG mutations at certain rare sites can be exceptions to the specific action of UAA and UAG suppressors. Some of the mutants appeared to require certain types of amino acid replacements at the mutant sites in order to produce a functional gene product, while others appeared to require suppressors that were expressed at high levels. Many of the mutants suppressible by SUP46 and paromomycin were not suppressible by any of the UAA, UAG or UGA suppressors, indicating that omnipotent suppression and phenotypic suppression need not be restricted to nonsense mutations. All of the mutants suppressible by SUP46 were also suppressible by paromomycin, suggesting a common mode of action of omnipotent suppression and phenotypic misreading.
Submitted on April 19, 1979
This article has been cited by other articles:
 |
|
 |
|
  B. Das, S. Das, and F. Sherman Mutant LYS2 mRNAs retained and degraded in the nucleus of Saccharomyces cerevisiae PNAS, July 18, 2006; 103(29): 10871 - 10876. [Abstract] [Full Text] [PDF]
|
|