EVIDENCE FOR CO-DOMINANCE OF THE HOMOTHALLIC GENES, HMalpha/hmalpha AND HMa/hma, IN SACCHAROMYCES YEASTS

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EVIDENCE FOR CO-DOMINANCE OF THE HOMOTHALLIC GENES, HM $alpha$ /hm $alpha$ AND HMa/hma, IN SACCHAROMYCES YEASTS

Kenji Arima ¹ and Isamu Takano ¹

¹ The Central Research Institute, Suntory Ltd., Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618, Japan

To investigate the dominance and recessiveness of the homothallismgenes, HM $alpha$ /hm $alpha$ and HMa/hma, for mating-type conversion, we constructedhybrids with various configurations of the homothallic genesby fusion of protoplasts prepared from haploid strains havingidentical mating types. Eight different combinations of thehomothallic genes were tested for their function by observingthe mating and sporulation abilities of the fusion products.With few exceptions, nonmating and sporogenous fusion productswere obtained from the following combinations: $alpha$ HO hm $alpha$ HMa + $alpha$ ho hm $alpha$ hma, $alpha$ HO hm $alpha$ HMa + $alpha$ ho HM $alpha$ hma, $alpha$ HO hm $alpha$ HMa + $alpha$ ho HM $alpha$ HMa,a HO HM $alpha$ hma + a ho hm $alpha$ hma, a HO HM $alpha$ hma + a ho hm $alpha$ HMa and a HOHM $alpha$ hma + a ho HM $alpha$ HMa. All the fusion products from the $alpha$ HO hm $alpha$ HMa + $alpha$ ho hm $alpha$ HMa and a HO HM $alpha$ hma + a ho HM $alpha$ hma combinationsshowed mating types identical to those of the respective haploidstrains. These results clearly support the co-dominance of theHM $alpha$ /hm $alpha$ and HMa/hma alleles and indicate that the hm $alpha$ allele hasthe same function as the HMa allele and that the hma allelehas the same function as the HM $alpha$ allele.

Submitted on February 5, 1979

EVIDENCE FOR CO-DOMINANCE OF THE HOMOTHALLIC GENES, HM/hm AND HMa/hma, IN SACCHAROMYCES YEASTS

Kenji Arima 1 and Isamu Takano 1

EVIDENCE FOR CO-DOMINANCE OF THE HOMOTHALLIC GENES, HM $alpha$ /hm $alpha$ AND HMa/hma, IN SACCHAROMYCES YEASTS

Kenji Arima ¹ and Isamu Takano ¹