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ESCHERICHIA COLI K-12 AUXOTROPHS INDUCED BY INSERTION OF THE TRANSPOSABLE ELEMENT Tn5
Karen J. Shaw 1 and Claire M. Berg 1
1 Biological Sciences Group (BoxU-131), The University of Connecticut, Storrs, Connecticut 06268
The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although mutations in at least 45 different cistrons were obtained, the distribution of insertion sites was not completely random: proA or proB; cysG; and cysH, cysD or cysC mutants were found in excess.
Submitted on October 5, 1978Revised on March 7, 1979
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