ULTRAVIOLET-INDUCED REVERSION OF cyc1 ALLELES IN RADIATION-SENSITIVE STRAINS OF YEAST. III. rev3 MUTANT STRAINS

Christopher W. Lawrence ¹ and Roshan B. Christensen ²

¹ Department of Radiation Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642
² Department of Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642

The role of the REV3 gene function in UV-induced mutagensis in the yeast Saccharomyces cerevisiae has been examined by determining the reversion of 12 well-defined cyc1 mutations in diploid strains homozygous for the rev3–1 or rev3–3 allele. The 12 cyc1 alleles include one ochre, one amber, four initiation, two proline missense, and four frameshift mutations. We find that the rev3 mutations reduce the frequency of UV-induced reversion of all of the cyc1 alleles, though different classes of alleles respond to a different extent. These results imply that the REV3 gene function is required for the production of a wide variety of mutational events, though probably not all, and show that each of the three REV loci have different mutational phenotypes. Such diverse phenotypes are not predicted by the unitary model for bacterial mutagenesis (Caillet-Fauquet, Defais and Radman 1977; Witkin 1976), suggesting that this is at best an incomplete description of eukaryotic mutagenesis.

This article has been cited by other articles:

				E. Mito, J. V. Mokhnatkin, M. C. Steele, V. L. Buettner, S. S. Sommer, G. M. Manthey, and A. M. Bailis Mutagenic and Recombinagenic Responses to Defective DNA Polymerase {delta} Are Facilitated by the Rev1 Protein in pol3-t Mutants of Saccharomyces cerevisiae Genetics, August 1, 2008; 179(4): 1795 - 1806. [Abstract] [Full Text] [PDF]

				C. A. Howell, C. M. Kondratick, and M. T. Washington Substitution of a residue contacting the triphosphate moiety of the incoming nucleotide increases the fidelity of yeast DNA polymerase {zeta} Nucleic Acids Res., March 1, 2008; 36(5): 1731 - 1740. [Abstract] [Full Text] [PDF]

				K. Sugasawa Xeroderma pigmentosum genes: functions inside and outside DNA repair Carcinogenesis, March 1, 2008; 29(3): 455 - 465. [Abstract] [Full Text] [PDF]

				N. Acharya, L. Haracska, S. Prakash, and L. Prakash Complex Formation of Yeast Rev1 with DNA Polymerase {eta} Mol. Cell. Biol., December 1, 2007; 27(23): 8401 - 8408. [Abstract] [Full Text] [PDF]

				V. Gangavarapu, S. Prakash, and L. Prakash Requirement of RAD52 Group Genes for Postreplication Repair of UV-Damaged DNA in Saccharomyces cerevisiae Mol. Cell. Biol., November 1, 2007; 27(21): 7758 - 7764. [Abstract] [Full Text] [PDF]

				N. Acharya, R. E. Johnson, S. Prakash, and L. Prakash Complex Formation with Rev1 Enhances the Proficiency of Saccharomyces cerevisiae DNA Polymerase {zeta} for Mismatch Extension and for Extension Opposite from DNA Lesions Mol. Cell. Biol., December 15, 2006; 26(24): 9555 - 9563. [Abstract] [Full Text] [PDF]

				N. Acharya, L. Haracska, R. E. Johnson, I. Unk, S. Prakash, and L. Prakash Complex Formation of Yeast Rev1 and Rev7 Proteins: a Novel Role for the Polymerase-Associated Domain Mol. Cell. Biol., November 1, 2005; 25(21): 9734 - 9740. [Abstract] [Full Text] [PDF]

				Y. Guo, L. L. Breeden, H. Zarbl, B. D. Preston, and D. L. Eaton Expression of a Human Cytochrome P450 in Yeast Permits Analysis of Pathways for Response to and Repair of Aflatoxin-Induced DNA Damage Mol. Cell. Biol., July 15, 2005; 25(14): 5823 - 5833. [Abstract] [Full Text] [PDF]

				T. Okada, E. Sonoda, M. Yoshimura, Y. Kawano, H. Saya, M. Kohzaki, and S. Takeda Multiple Roles of Vertebrate REV Genes in DNA Repair and Recombination Mol. Cell. Biol., July 15, 2005; 25(14): 6103 - 6111. [Abstract] [Full Text] [PDF]

				M. Diaz, N. B. Watson, G. Turkington, L. K. Verkoczy, N. R. Klinman, and W. G. McGregor Decreased Frequency and Highly Aberrant Spectrum of Ultraviolet-Induced Mutations in the hprt Gene of Mouse Fibroblasts Expressing Antisense RNA to DNA Polymerase {zeta} Mol. Cancer Res., September 1, 2003; 1(11): 836 - 847. [Abstract] [Full Text] [PDF]

				A. Sakamoto, V. T. T. Lan, Y. Hase, N. Shikazono, T. Matsunaga, and A. Tanaka Disruption of the AtREV3 Gene Causes Hypersensitivity to Ultraviolet B Light and {gamma}-Rays in Arabidopsis: Implication of the Presence of a Translesion Synthesis Mechanism in Plants PLANT CELL, September 1, 2003; 15(9): 2042 - 2057. [Abstract] [Full Text] [PDF]

				A. J. Rattray, B. K. Shafer, C. B. McGill, and J. N. Strathern The Roles of REV3 and RAD57 in Double-Strand-Break-Repair-Induced Mutagenesis of Saccharomyces cerevisiae Genetics, November 1, 2002; 162(3): 1063 - 1077. [Abstract] [Full Text] [PDF]

				S. Broomfield and W. Xiao Suppression of genetic defects within the RAD6 pathway by srs2 is specific for error-free post-replication repair but not for damage-induced mutagenesis Nucleic Acids Res., February 1, 2002; 30(3): 732 - 739. [Abstract] [Full Text] [PDF]

				P. E. M. Gibbs, W. G. McGregor, V. M. Maher, P. Nisson, and C. W. Lawrence A human homolog of the Saccharomyces cerevisiae REV3 gene, which encodes the catalytic subunit of DNA polymerase zeta PNAS, June 9, 1998; 95(12): 6876 - 6880. [Abstract] [Full Text] [PDF]

				Y. Masuda, M. Takahashi, N. Tsunekuni, T. Minami, M. Sumii, K. Miyagawa, and K. Kamiya Deoxycytidyl Transferase Activity of the Human REV1 Protein Is Closely Associated with the Conserved Polymerase Domain J. Biol. Chem., April 27, 2001; 276(18): 15051 - 15058. [Abstract] [Full Text] [PDF]