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A CLUSTER OF THREE GENES RESPONSIBLE FOR ALLANTOIN DEGRADATION IN SACCHAROMYCES CEREVISIAE
Terrance G. Cooper 1, Mary Gorski 1, and Vanessa Turoscy 1
1 Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260
Mutant strains of Saccharomyces cerevisiae unable to utilize allantoin as sole nitrogen source were isolated and divided into three groups on the basis of their biochemical and genetic characteristics. The three loci associated with these mutant classes were designated dal1 (allantoinase minus), dal2 (allantoicase minus) and dal4 (allantoin transport minus). All three loci are located in a cluster that is proximal to the lys1 locus on the right arm of chromosome IX. The gene order and intergenic distances were estimated to be: dal1—2.5 cM—dal4—1.9 cM—dal2—4.6 cM—lys1.
Submitted on September 8, 1978Revised on December 27, 1978
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