THE CAN1 LOCUS OF SACCHAROMYCES CEREVISIAE: FINE-STRUCTURE ANALYSIS AND FORWARD MUTATION RATES

William L. Whelan ¹, Elmar Gocke ¹, and Thomas R. Manney ¹

¹ Department of Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 and Department of Physics, Kansas State University, Manhattan, Kansas 66506

A system of strains and growth media was developed to allow efficient detection of forward mutation, reversion, complementation, and suppression at the canavanine-resistance (CAN1) locus of Saccharomyces cerevisiae. Genetic fine-structure analysis revealed that the map length is at least 40, and possibly as much as 60 X-ray map units; this is the longest gene map yet reported in S. cerevisiae. Allelic complementation was not observed, despite testing of a large number of allele pairs, and alleles suppressible by the ochre suppressor SUP11 were absent from a sample of 48 spontaneous mutants and occurred infrequently (7%) among a sample of ultraviolet-induced mutants. Infrequent mutant types included canavanine-resistant mutants capable of arginine uptake and alleles thought to represent deletions or inversions. In contrast to previous reports in the literature, the spontaneous forward mutation rate at CAN1 did not increase during meiosis.

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