A CHROMOSOMAL TRANSLOCATION CAUSING OVERPRODUCTION OF ISO-2-CYTOCHROME c IN YEAST

Fred Sherman ¹ and Cynthia Helms ²

¹ Department of Radiation Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642
² Department of Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642

The CYC7–1 mutation in the yeast Saccharomyces cerevisiae causes the production of approximately 30 times the normal amount of iso-2-cytochrome c. Genetic analysis established that the CYC7–1 mutation is a reciprocal translocation involving the left arm of chromosome V and the right arm of chromosome XVI. The chromosome V arm was broken adjacent to the gene CYC7, which determines the primary structure of iso-2-cytochrome c, and this fragment containing the CYC7 gene was joined to the segment of chromosome XVI. It appears as though the elevation of iso-2-cytochrome c is caused by an abnormal controlling region adjacent to the structural region of the CYC7 gene.

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