GENETIC LENGTHS AND BREAK POINTS IN TWELVE CHROMOSOMES OF GOSSYPIUM HIRSUTUM INVOLVED IN TEN RECIPROCAL TRANSLOCATIONS

Margaret Y. Menzel ¹ and Meta S. Brown ²

¹ Department of Biological Sciences, Florida State University, Tallahassee, Florida 32306
² Department of Soil and Crop Sciences, Texas A&M University, College Station, Texas

Chromosome configurations were recorded in about 5500 pollen mother cells (PMC's) in 2n and 2n-1 (missing the intact A-genome chromosome) heterozygotes of ten reciprocal translocations involving six A-genome chromosomes (H1, H2, H3, H4, H6 and H7) and six D-genome chromosomes (H14, H15, H16, H19, H20 and H21) of Gossypium hirsutum. From these records, chiasma frequencies at each of six positions were determined for nine translocations and at two positions for one. These frequencies were used to calculate recombination frequencies in different chromosome regions, and from these distances the breakpoints in 15 chromosome arms were mapped relative to each other and to their respective centromeres, insofar as the data permitted. The karyotype so derived for twelve chromosomes is in reasonably good agreement with data from genetic mapping, telosome and monosome mapping, and the mitotic idiogram.