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CONTEXT EFFECTS ON NONSENSE CODON SUPPRESSION IN ESCHERICHIA COLI
Sheldon I. Feinstein 1 and Sidney Altman 1
1 Department of Biology, Yale University, New Haven, Connecticut
06520
The influence of mRNA context on nonsense codon suppression has been studied by suppression measurements at one site in the Escherichia coli trpE gene and at two sites in the trpA gene. The ratio of suppression efficiencies of amber and ochre codons at each site (homotopic pairs) has been compared using ochre suppressing derivatives of tRNA 1Tyr. This ratio is independent of differential effects of the inserted amino acid on enzyme function. We have found that mRNA context can change the ratio of suppression efficiencies of homotopic nonsense codons at the three sites in the trp gene system over a ten-fold range. The causes of such variation, and, in particular the effect of certain adjacent nucleotides on nonsense codon suppression are considered.
Submitted on June 30, 1977Revised on September 12, 1977
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