GLYCOLYSIS MUTANTS IN SACCHAROMYCES CEREVISIAE

Drago Clifton ¹, Shelley B. Weinstock ¹, and Dan G. Fraenkel ¹

¹ Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

Mutants have been isolated in S. cerevisiae with the phenotype of growth on pyruvate but not on glucose, or growth on rich medium with pyruvate but inhibition by glucose. Screening of mutagenized cultures was either without an enrichment step, or after enrichment using the antibiotic netropsin (Young et al. 1976) or inositol starvation ( Henry, Donahue and Culbertson 1975). One class of mutants lacked pyruvate kinase (pyk), another class had all the enzymes of glycolysis, and one mutant lacked phosphoglucose isomerase (pgi, Maitra 1971). Partial reversion of pyruvate kinase mutants on rich medium containing glucose gave double mutants now also lacking hexokinase (hxk ), phosphofructokinase (pfk), or several enzymes of glycolysis (gcr). In diploids the mutations were recessive. pyk, pgi, pfk , and gcr segregated 2:2 from their wild-type alleles. PYK hxk, PYK pfk, and PYK gcr segregants grew on glucose.

This article has been cited by other articles:

				K. Tanneberger, J. Kirchberger, J. Bar, W. Schellenberger, S. Rothemund, M. Kamprad, H. Otto, T. Schoneberg, and A. Edelmann A Novel Form of 6-Phosphofructokinase: IDENTIFICATION AND FUNCTIONAL RELEVANCE OF A THIRD TYPE OF SUBUNIT IN PICHIA PASTORIS J. Biol. Chem., August 10, 2007; 282(32): 23687 - 23697. [Abstract] [Full Text] [PDF]

				G. M. Santangelo Glucose Signaling in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 253 - 282. [Abstract] [Full Text] [PDF]

				M. Lemaire and M. Wesolowski-Louvel Enolase and Glycolytic Flux Play a Role in the Regulation of the Glucose Permease Gene RAG1 of Kluyveromyces lactis Genetics, October 1, 2004; 168(2): 723 - 731. [Abstract] [Full Text] [PDF]

				I. Famili, J. Forster, J. Nielsen, and B. O. Palsson Saccharomyces cerevisiae phenotypes can be predicted by using constraint-based analysis of a genome-scale reconstructed metabolic network PNAS, November 11, 2003; 100(23): 13134 - 13139. [Abstract] [Full Text] [PDF]

				M. C. López and H. V. Baker Understanding the Growth Phenotype of the Yeast gcr1 Mutant in Terms of Global Genomic Expression Patterns J. Bacteriol., September 1, 2000; 182(17): 4970 - 4978. [Abstract] [Full Text]

				J. J. Kang, R. M. Watson, M. E. Fisher, R. Higuchi, D. H. Gelfand, and M. J. Holland Transcript quantitation in total yeast cellular RNA using kinetic PCR Nucleic Acids Res., January 15, 2000; 28(2): e2 - e2. [Abstract] [Full Text] [PDF]

				J. S. Hardwick, F. G. Kuruvilla, J. K. Tong, A. F. Shamji, and S. L. Schreiber Rapamycin-modulated transcription defines the subset of nutrient-sensitive signaling pathways directly controlled by the Tor proteins PNAS, December 21, 1999; 96(26): 14866 - 14870. [Abstract] [Full Text] [PDF]

				M. C. Lopez, J. B. Smerage, and H. V. Baker Multiple domains of repressor activator protein 1 contribute to facilitated binding of glycolysis regulatory protein 1 PNAS, November 24, 1998; 95(24): 14112 - 14117. [Abstract] [Full Text] [PDF]

				J. J. Heinisch, E. Boles, and C. Timpel A Yeast Phosphofructokinase Insensitive to the Allosteric Activator Fructose 2,6-Bisphosphate. GLYCOLYSIS/METABOLIC REGULATION/ALLOSTERIC CONTROL J. Biol. Chem., July 5, 1996; 271(27): 15928 - 15933. [Abstract] [Full Text] [PDF]

				K. Arndt, C Styles, and G. Fink Multiple global regulators control HIS4 transcription in yeast Science, August 21, 1987; 237(4817): 874 - 880. [Abstract] [PDF]

				R. Rodicio, A. Strau{beta}, and J. J. Heinisch Single Point Mutations in Either Gene Encoding the Subunits of the Heterooctameric Yeast Phosphofructokinase Abolish Allosteric Inhibition by ATP J. Biol. Chem., December 22, 2000; 275(52): 40952 - 40960. [Abstract] [Full Text] [PDF]