THE NUMBER OF CHROMOSOMES IN SCHIZOSACCHAROMYCES POMBE: LIGHT MICROSCOPY OF STAINED PREPARATIONS

C. F. Robinow ¹

¹ Department of Bacteriology and Immunology, University of Western Ontario, London, Canada

Chromosomes have been counted with the light microscope in fixed and stained preparations of Schizosaccharomyces pombe. The least ambiguous images were seen in zygotes fixed at meta-, ana- and telophase of meiosis II. They suggest that the haploid number of chromosomes in S. pombe is three.

This article has been cited by other articles:

				X. Peng, R. K. M. Karuturi, L. D. Miller, K. Lin, Y. Jia, P. Kondu, L. Wang, L.-S. Wong, E. T. Liu, M. K. Balasubramanian, et al. Identification of Cell Cycle-regulated Genes in Fission Yeast Mol. Biol. Cell, March 1, 2005; 16(3): 1026 - 1042. [Abstract] [Full Text] [PDF]

				A. Yamashita, M. Sato, A. Fujita, M. Yamamoto, and T. Toda The Roles of Fission Yeast Ase1 in Mitotic Cell Division, Meiotic Nuclear Oscillation, and Cytokinesis Checkpoint Signaling Mol. Biol. Cell, March 1, 2005; 16(3): 1378 - 1395. [Abstract] [Full Text] [PDF]

				M. Molnar, E. Doll, A. Yamamoto, Y. Hiraoka, and J. Kohli Linear element formation and their role in meiotic sister chromatid cohesion and chromosome pairing J. Cell Sci., May 1, 2003; 116(9): 1719 - 1731. [Abstract] [Full Text] [PDF]

				Y. Jin, S. Uzawa, and W. Z. Cande Fission Yeast Mutants Affecting Telomere Clustering and Meiosis-Specific Spindle Pole Body Integrity Genetics, March 1, 2002; 160(3): 861 - 876. [Abstract] [Full Text] [PDF]

				S. Rajagopalan and M. K. Balasubramanian Schizosaccharomyces pombe Bir1p, a Nuclear Protein That Localizes to Kinetochores and the Spindle Midzone, Is Essential for Chromosome Condensation and Spindle Elongation During Mitosis Genetics, February 1, 2002; 160(2): 445 - 456. [Abstract] [Full Text] [PDF]

				M. Molnar, J. Bahler, J. Kohli, and Y. Hiraoka Live observation of fission yeast meiosis in recombination-deficient mutants: a study on achiasmate chromosome segregation J. Cell Sci., January 8, 2001; 114(15): 2843 - 2853. [Abstract] [Full Text] [PDF]

				S. Shinozaki-Yabana, Y. Watanabe, and M. Yamamoto Novel WD-Repeat Protein Mip1p Facilitates Function of the Meiotic Regulator Mei2p in Fission Yeast Mol. Cell. Biol., February 15, 2000; 20(4): 1234 - 1242. [Abstract] [Full Text]

				H. Murakami and P. Nurse Meiotic DNA replication checkpoint control in fission yeast Genes & Dev., October 1, 1999; 13(19): 2581 - 2593. [Abstract] [Full Text]

				E. Hartsuiker, J. Bähler, and J. Kohli The Role of Topoisomerase II in Meiotic Chromosome Condensation and Segregation in Schizosaccharomyces pombe Mol. Biol. Cell, October 1, 1998; 9(10): 2739 - 2750. [Abstract] [Full Text]

				S. Horie, Y. Watanabe, K. Tanaka, S. Nishiwaki, H. Fujioka, H. Abe, M. Yamamoto, and C. Shimoda The Schizosaccharomyces pombe mei4+ Gene Encodes a Meiosis-Specific Transcription Factor Containing a forkhead DNA-Binding Domain Mol. Cell. Biol., April 1, 1998; 18(4): 2118 - 2129. [Abstract] [Full Text]

				Y. Tange, T. Horio, M. Shimanuki, D.-Q. Ding, Y. Hiraoka, and O. Niwa A Novel Fission Yeast Gene, tht1+, Is Required for the Fusion of Nuclear Envelopes during Karyogamy J. Cell Biol., January 26, 1998; 140(2): 247 - 258. [Abstract] [Full Text] [PDF]

				J Petersen, O Nielsen, R Egel, and I. Hagan F-actin distribution and function during sexual differentiation in Schizosaccharomyces pombe J. Cell Sci., January 4, 1998; 111(7): 867 - 876. [Abstract] [PDF]

				D. Ding, Y Chikashige, T Haraguchi, and Y Hiraoka Oscillatory nuclear movement in fission yeast meiotic prophase is driven by astral microtubules, as revealed by continuous observation of chromosomes and microtubules in living cells J. Cell Sci., January 3, 1998; 111(6): 701 - 712. [Abstract] [PDF]

				Y Chikashige, D. Ding, H Funabiki, T Haraguchi, S Mashiko, M Yanagida, and Y Hiraoka Telomere-led premeiotic chromosome movement in fission yeast Science, April 8, 1994; 264(5156): 270 - 273. [Abstract] [PDF]