VIRUS-LIKE PARTICLES IN USTILAGO MAYDIS: MUTANTS WITH PARTIAL GENOMES

1 Department of Microbiology, George S. Wise Center for Life Sciences, Tel Aviv University, Ramat Aviv, Israel

Mutants with partial genomes for the virus-like particles of U. maydis were recovered following treatment with nitrosoguanidine. Examination of the properties retained by progeny of genetic crosses indicates that the 2.9 x 106 dalton component of double-stranded RNA contains the information for capsid formation and dsRNA replication. Other components appear to contain the information for killer function and immunity to killer. The use of such mutants for studies on the evolution of viruses with segmented genomes is discussed.

Submitted on January 4, 1977
Revised on February 28, 1977