EFFECTS OF THE MITOTIC CELL-CYCLE MUTATION cdc4 ON YEAST MEIOSIS

G. Simchen ¹ and J. Hirschberg ¹

¹ Department of Genetics, The Hebrew University, Jerusalem, Israel

The mitotic cell-cycle mutation cdc4 has been reported to block the initiation of nuclear DNA replication and the separation of spindle plaques after their replication. Meiosis in cdc4/cdc4 diploids is normal at the permissive temperature (25°) and is arrested at the first division (one-nucleus stage) at the restrictive temperature (34° or 36°). Arrested cells at 34° show a high degree of commitment to recombination (at least 50% of the controls) but no haploidization, while cells arrested at 36° are not committed to recombination. Meiotic cells arrested at 34° show a delayed and reduced synthesis of DNA (at most 40% of the control), at least half of which is probably mitochondrial. It is suggested that recombination commitment does not depend on the completion of nuclear premeiotic DNA replication in sporulation medium.—Transfer of cdc4/cdc4 cells to the restrictive temperature at the onset of sporulation produces a uniform phenotype of arrest at a 1-nucleus morphology. On the other hand, shifts of the meiotic cells to the restrictive temperature at later times produce two additional phenotypes of arrest, thus suggesting that the function of cdc4 is required at several points in meiosis (at least at three different times).

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