A GENETIC FINE STRUCTURE ANALYSIS OF THE SUPPRESSOR 3 LOCUS IN SACCHAROMYCES

1 Committee on Genetics and Department of Biology, University of Chicago, Chicago, Illinois 60637

A meiotic fine structure map of a yeast tyrosine-inserting ochre suppressor, SUP3-o, was constructed. This was accomplished by examining ten intragenic suppressor-inactive revertants for their relationship to each other and to the original SUP3-o mutation. The second-site revertants map on both sides of the SUP3-o mutation. The meiotic map length based on the summation of short intervals is 45+/10 5 asci.

Submitted on March 9, 1976
Revised on September 15, 1976




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D. Burke, G. Carle, and M. Olson
Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors
Science, May 15, 1987; 236(4803): 806 - 812.
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