PROTEINASE MUTANTS OF SACCHAROMYCES CEREVISIAE

Elizabeth W. Jones ¹

¹ Department of Biological Sciences, Mellon Institute of Science, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213

Fifty-nine mutants with reduced ability to cleave the chymotrypsin substrate N-acetyl-DL-phenylalanine ß-naphthyl ester have been isolated in S. cerevisiae. All have reduced levels of one or more of the three well-characterized proteinases in yeast. All have reduced levels of proteinase C (carboxy-peptidase Y). These mutations define 16 complementation groups.

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