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MAPPING OF THE HOMOTHALLIC GENES, HM
AND
HMa, IN SACCHAROMYCES YEASTS
Satoshi Harashima 1 and Yasuji Oshima 1
1 Department of Fermentation Technology, Osaka University, Yamadakami,
Suita-shi, Osaka 565, Japan
Two of the three homothallic genes, HM
and
HMa, showed direct linkage to the mating-type locus at approximately
73 and 98 stranes (57 and 65 centimorgans [cM]), respectively, whereas, the
other, HO, showed no linkage to 25 standard markers distributed over
17 chromosomes including the mating-type locus. To determine whether the
HM and HMa loci located on the left or right side
of the mating-type locus, equations for three factor analysis of three linked
genes were derived. Tetrad data were collected and were compared with expected
values by 
2 statistics. Calculations indicated that the
HM gene is probably located on the right arm at 95 stranes (65
cM) from the centromere and the HMa locus at approximately
90 stranes (64 cM) on the left arm of chromosome III.
Revised on July 16, 1976
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