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MAPPING OF TRICHODERMIN RESISTANCE IN SACCHAROMYCES CEREVISIAE : A GENETIC LOCUS FOR A COMPONENT OF THE 60S RIBSOMAL SUBUNIT
Paul G. Grant 1, Daniel Schindler 1, and Julian E. Davies 1
1 Department of Biochemistry, College of Agricultural and Life
Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706
Resistance to the protein synthesis inhibitor trichodermin in Saccharomyces cerevisiae has been studied. A single recessive nuclear gene was responsible for resistance. The resistance locus, tcm1 was found to be closely linked (1 centimorgan) to the locus pet 17 on the right arm of chromosome XV. The mutation to trichodermin resistance conferred resistance to other 12,13-epoxytrichothecenes and to the structurally unrelated antibiotic anisomycin.
Submitted on December 29, 1975Revised on April 6, 1976
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