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GALACTOKINASE MUTANTS OF CHINESE HAMSTER SOMATIC CELLS RESISTANT TO 2-DEOXYGALACTOSE
Jean-Paul Thirion 1, Denis Banville 1, and Henri Noel 1
1 Département de Microbiologie, Centre Hospitalier Universitaire,
Université de Sherbrooke, P.Q. Canada J1H 5N4
The growth of Chinese hamster somatic cells was inhibited by 0.2 mg/cc of 2-deoxygalactose. Mutants partially or fully resistant to 2-deoxygalactose were isolated in a single-step or two-step selection. Some of them did not grow as well as the wild type; one of them which lacked galactokinase (EC.2.7.1.6) activity did not grow at all in galactose medium. The galactokinase kinetic properties (Vmax & Kmax) of the other mutants and of the wild type were different. Therefore resistance resulted either from the possible absence of galactokinase synthesis or from a structural mutation, possibly a missence mutation, in the galactokinase gene.—A simple diagnostic test for juvenile cataract is proposed.
Revised on January 19, 1976