LAMBDA CIN-1, A NEW MUTATION WHICH ENHANCES LYSOGENIZATION BY BACTERIOPHAGE LAMBDA, AND THE GENETIC STRUCTURE OF THE LAMBDA CY REGION

Daniel L. Wulff ¹

¹ Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, California 92717

Seven lambda cy mutants have been mapped within a small region located approximately halfway between the rightward boundary of the imm⁴³⁴ region and the lambda cII gene. The seven mutants lie at four sites separated by a total distance of about 12 nucleotide pairs, as estimated from recombination frequencies. Six of the seven mutants lie on the right side of the cy fine structure map, spanning a total distance of about 3–5 nucleotide pairs. Lying approximately 11–21 nucleotide pairs to the left of the leftmost cy mutant is a newly described mutation called cin-1, for c independent. The cin-1 mutation allows some lysogenization when coupled with any cy, cII or cIII mutant, but not when coupled with a defective cI gene. The cin-1 mutation, like cy mutants, has a cis-dominant action upon the cI gene in mixed infections. The observation that imm⁴³⁴ cin-1 cy2001 lysogenizes efficiently, but not imm⁴³⁴ cin-1 cy2001 cII68 nor any other imm⁴³⁴ cin-1 cy derivative, is interpreted to mean that all of the cy mutants on the right side of the cy fine structure map inactivate a binding site for cII/cIII function, but that cy2001, the single mutant on the left side of the cy fine structure map, does not inactivate that binding site.

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