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MUTANT ENRICHMENT IN THE COLONIAL ALGA, EUDORINA ELEGANS
A. L. Toby 1 and C. L. Kemp 1
1 Department of Biological Sciences, Simon Fraser University,
Burnaby, Canada V5A 1S6
An enrichment procedure has been developed that results in at least a 200x increase in mutation frequency in the colonial alga, Eudorina elegans. A period of nitrogen starvation followed by treatment with 8-azaguanine results in the death of wild-type cells and the maintenance of mutants. N'-nitro-N-nitro-soguanidine-induced acetate, p-aminobenzoic acid and reduced nitrogen requiring mutants have been isolated by this procedure.
Submitted on March 3, 1975Revised on May 14, 1975