A DELETION MAP OF cyc1 MUTANTS AND ITS CORRESPONDENCE TO MUTATIONALLY ALTERED ISO-1-CYTOCHROMES c OF YEAST

Fred Sherman ¹, Mary Jackson ¹, Susan W. Liebman ¹, Ann Marie Schweingruber ¹, and John W. Stewart ¹

¹ Department of Radiation Biology and Biophysics University of Rochester School of Medicine and Dentistry, Rochester, New York 14642

Mutants arising spontaneously from sporulated cultures of certain strains of yeast, Saccharomyces cerevisiae, contained deletions of the CYC1 gene which controls the primary structure of iso-1-cytochrome c. At least 60 different kinds of deletions were uncovered among the 104 deletions examined and these ranged in length from those encompassing only two adjacent point mutants to those encompassing at least the entire CYC1 gene. X-ray-induced recombination rates of crosses involving these deletions and cyc1 point mutants resulted in the assignment of 211 point mutants to 47 mutational sites and made it possible to unambiguously order 40 of these 47 sites. Except for one mutant, cyc1-15, there was a strict colinear relationship between the deletion map and the positions of 13 sites that were previously determined by amino acid alterations in iso-1-cytochromes c from intragenic revertants.

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