CHEMOTAXIS-DEFECTIVE MUTANTS OF THE NEMATODE CAENORHABDITIS ELEGANS

David B. Dusenbery ¹, Robert E. Sheridan ¹, and Richard L. Russell ¹

¹ Division of Biology, California Institute of Technology, Pasadena, California 91125

The technique of countercurrent separation has been used to isolate 17 independent chemotaxis-defective mutants of the nematode Caenorhabditis elegans. The mutants, selected to be relatively insensitive to the normally attractive salt NaCl, show varying degrees of residual sensitivity; some are actually weakly repelled by NaCl. The mutants are due to single gene defects, are autosomal and recessive, and identify at least five complementation groups.

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